examples of conclusion research paper

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How to Write a Conclusion for Research Papers (with Examples)

How to Write a Conclusion for Research Papers (with Examples)

The conclusion of a research paper is a crucial section that plays a significant role in the overall impact and effectiveness of your research paper. However, this is also the section that typically receives less attention compared to the introduction and the body of the paper. The conclusion serves to provide a concise summary of the key findings, their significance, their implications, and a sense of closure to the study. Discussing how can the findings be applied in real-world scenarios or inform policy, practice, or decision-making is especially valuable to practitioners and policymakers. The research paper conclusion also provides researchers with clear insights and valuable information for their own work, which they can then build on and contribute to the advancement of knowledge in the field.

The research paper conclusion should explain the significance of your findings within the broader context of your field. It restates how your results contribute to the existing body of knowledge and whether they confirm or challenge existing theories or hypotheses. Also, by identifying unanswered questions or areas requiring further investigation, your awareness of the broader research landscape can be demonstrated.

Remember to tailor the research paper conclusion to the specific needs and interests of your intended audience, which may include researchers, practitioners, policymakers, or a combination of these.

Table of Contents

What is a conclusion in a research paper, summarizing conclusion, editorial conclusion, externalizing conclusion, importance of a good research paper conclusion, how to write a conclusion for your research paper, research paper conclusion examples.

  • How to write a research paper conclusion with Paperpal? 

Frequently Asked Questions

A conclusion in a research paper is the final section where you summarize and wrap up your research, presenting the key findings and insights derived from your study. The research paper conclusion is not the place to introduce new information or data that was not discussed in the main body of the paper. When working on how to conclude a research paper, remember to stick to summarizing and interpreting existing content. The research paper conclusion serves the following purposes: 1

  • Warn readers of the possible consequences of not attending to the problem.
  • Recommend specific course(s) of action.
  • Restate key ideas to drive home the ultimate point of your research paper.
  • Provide a “take-home” message that you want the readers to remember about your study.

examples of conclusion research paper

Types of conclusions for research papers

In research papers, the conclusion provides closure to the reader. The type of research paper conclusion you choose depends on the nature of your study, your goals, and your target audience. I provide you with three common types of conclusions:

A summarizing conclusion is the most common type of conclusion in research papers. It involves summarizing the main points, reiterating the research question, and restating the significance of the findings. This common type of research paper conclusion is used across different disciplines.

An editorial conclusion is less common but can be used in research papers that are focused on proposing or advocating for a particular viewpoint or policy. It involves presenting a strong editorial or opinion based on the research findings and offering recommendations or calls to action.

An externalizing conclusion is a type of conclusion that extends the research beyond the scope of the paper by suggesting potential future research directions or discussing the broader implications of the findings. This type of conclusion is often used in more theoretical or exploratory research papers.

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The conclusion in a research paper serves several important purposes:

  • Offers Implications and Recommendations : Your research paper conclusion is an excellent place to discuss the broader implications of your research and suggest potential areas for further study. It’s also an opportunity to offer practical recommendations based on your findings.
  • Provides Closure : A good research paper conclusion provides a sense of closure to your paper. It should leave the reader with a feeling that they have reached the end of a well-structured and thought-provoking research project.
  • Leaves a Lasting Impression : Writing a well-crafted research paper conclusion leaves a lasting impression on your readers. It’s your final opportunity to leave them with a new idea, a call to action, or a memorable quote.

examples of conclusion research paper

Writing a strong conclusion for your research paper is essential to leave a lasting impression on your readers. Here’s a step-by-step process to help you create and know what to put in the conclusion of a research paper: 2

  • Research Statement : Begin your research paper conclusion by restating your research statement. This reminds the reader of the main point you’ve been trying to prove throughout your paper. Keep it concise and clear.
  • Key Points : Summarize the main arguments and key points you’ve made in your paper. Avoid introducing new information in the research paper conclusion. Instead, provide a concise overview of what you’ve discussed in the body of your paper.
  • Address the Research Questions : If your research paper is based on specific research questions or hypotheses, briefly address whether you’ve answered them or achieved your research goals. Discuss the significance of your findings in this context.
  • Significance : Highlight the importance of your research and its relevance in the broader context. Explain why your findings matter and how they contribute to the existing knowledge in your field.
  • Implications : Explore the practical or theoretical implications of your research. How might your findings impact future research, policy, or real-world applications? Consider the “so what?” question.
  • Future Research : Offer suggestions for future research in your area. What questions or aspects remain unanswered or warrant further investigation? This shows that your work opens the door for future exploration.
  • Closing Thought : Conclude your research paper conclusion with a thought-provoking or memorable statement. This can leave a lasting impression on your readers and wrap up your paper effectively. Avoid introducing new information or arguments here.
  • Proofread and Revise : Carefully proofread your conclusion for grammar, spelling, and clarity. Ensure that your ideas flow smoothly and that your conclusion is coherent and well-structured.

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Remember that a well-crafted research paper conclusion is a reflection of the strength of your research and your ability to communicate its significance effectively. It should leave a lasting impression on your readers and tie together all the threads of your paper. Now you know how to start the conclusion of a research paper and what elements to include to make it impactful, let’s look at a research paper conclusion sample.

Summarizing ConclusionImpact of social media on adolescents’ mental healthIn conclusion, our study has shown that increased usage of social media is significantly associated with higher levels of anxiety and depression among adolescents. These findings highlight the importance of understanding the complex relationship between social media and mental health to develop effective interventions and support systems for this vulnerable population.
Editorial ConclusionEnvironmental impact of plastic wasteIn light of our research findings, it is clear that we are facing a plastic pollution crisis. To mitigate this issue, we strongly recommend a comprehensive ban on single-use plastics, increased recycling initiatives, and public awareness campaigns to change consumer behavior. The responsibility falls on governments, businesses, and individuals to take immediate actions to protect our planet and future generations.  
Externalizing ConclusionExploring applications of AI in healthcareWhile our study has provided insights into the current applications of AI in healthcare, the field is rapidly evolving. Future research should delve deeper into the ethical, legal, and social implications of AI in healthcare, as well as the long-term outcomes of AI-driven diagnostics and treatments. Furthermore, interdisciplinary collaboration between computer scientists, medical professionals, and policymakers is essential to harness the full potential of AI while addressing its challenges.

examples of conclusion research paper

How to write a research paper conclusion with Paperpal?

A research paper conclusion is not just a summary of your study, but a synthesis of the key findings that ties the research together and places it in a broader context. A research paper conclusion should be concise, typically around one paragraph in length. However, some complex topics may require a longer conclusion to ensure the reader is left with a clear understanding of the study’s significance. Paperpal, an AI writing assistant trusted by over 800,000 academics globally, can help you write a well-structured conclusion for your research paper. 

  • Sign Up or Log In: Create a new Paperpal account or login with your details.  
  • Navigate to Features : Once logged in, head over to the features’ side navigation pane. Click on Templates and you’ll find a suite of generative AI features to help you write better, faster.  
  • Generate an outline: Under Templates, select ‘Outlines’. Choose ‘Research article’ as your document type.  
  • Select your section: Since you’re focusing on the conclusion, select this section when prompted.  
  • Choose your field of study: Identifying your field of study allows Paperpal to provide more targeted suggestions, ensuring the relevance of your conclusion to your specific area of research. 
  • Provide a brief description of your study: Enter details about your research topic and findings. This information helps Paperpal generate a tailored outline that aligns with your paper’s content. 
  • Generate the conclusion outline: After entering all necessary details, click on ‘generate’. Paperpal will then create a structured outline for your conclusion, to help you start writing and build upon the outline.  
  • Write your conclusion: Use the generated outline to build your conclusion. The outline serves as a guide, ensuring you cover all critical aspects of a strong conclusion, from summarizing key findings to highlighting the research’s implications. 
  • Refine and enhance: Paperpal’s ‘Make Academic’ feature can be particularly useful in the final stages. Select any paragraph of your conclusion and use this feature to elevate the academic tone, ensuring your writing is aligned to the academic journal standards. 

By following these steps, Paperpal not only simplifies the process of writing a research paper conclusion but also ensures it is impactful, concise, and aligned with academic standards. Sign up with Paperpal today and write your research paper conclusion 2x faster .  

The research paper conclusion is a crucial part of your paper as it provides the final opportunity to leave a strong impression on your readers. In the research paper conclusion, summarize the main points of your research paper by restating your research statement, highlighting the most important findings, addressing the research questions or objectives, explaining the broader context of the study, discussing the significance of your findings, providing recommendations if applicable, and emphasizing the takeaway message. The main purpose of the conclusion is to remind the reader of the main point or argument of your paper and to provide a clear and concise summary of the key findings and their implications. All these elements should feature on your list of what to put in the conclusion of a research paper to create a strong final statement for your work.

A strong conclusion is a critical component of a research paper, as it provides an opportunity to wrap up your arguments, reiterate your main points, and leave a lasting impression on your readers. Here are the key elements of a strong research paper conclusion: 1. Conciseness : A research paper conclusion should be concise and to the point. It should not introduce new information or ideas that were not discussed in the body of the paper. 2. Summarization : The research paper conclusion should be comprehensive enough to give the reader a clear understanding of the research’s main contributions. 3 . Relevance : Ensure that the information included in the research paper conclusion is directly relevant to the research paper’s main topic and objectives; avoid unnecessary details. 4 . Connection to the Introduction : A well-structured research paper conclusion often revisits the key points made in the introduction and shows how the research has addressed the initial questions or objectives. 5. Emphasis : Highlight the significance and implications of your research. Why is your study important? What are the broader implications or applications of your findings? 6 . Call to Action : Include a call to action or a recommendation for future research or action based on your findings.

The length of a research paper conclusion can vary depending on several factors, including the overall length of the paper, the complexity of the research, and the specific journal requirements. While there is no strict rule for the length of a conclusion, but it’s generally advisable to keep it relatively short. A typical research paper conclusion might be around 5-10% of the paper’s total length. For example, if your paper is 10 pages long, the conclusion might be roughly half a page to one page in length.

In general, you do not need to include citations in the research paper conclusion. Citations are typically reserved for the body of the paper to support your arguments and provide evidence for your claims. However, there may be some exceptions to this rule: 1. If you are drawing a direct quote or paraphrasing a specific source in your research paper conclusion, you should include a citation to give proper credit to the original author. 2. If your conclusion refers to or discusses specific research, data, or sources that are crucial to the overall argument, citations can be included to reinforce your conclusion’s validity.

The conclusion of a research paper serves several important purposes: 1. Summarize the Key Points 2. Reinforce the Main Argument 3. Provide Closure 4. Offer Insights or Implications 5. Engage the Reader. 6. Reflect on Limitations

Remember that the primary purpose of the research paper conclusion is to leave a lasting impression on the reader, reinforcing the key points and providing closure to your research. It’s often the last part of the paper that the reader will see, so it should be strong and well-crafted.

  • Makar, G., Foltz, C., Lendner, M., & Vaccaro, A. R. (2018). How to write effective discussion and conclusion sections. Clinical spine surgery, 31(8), 345-346.
  • Bunton, D. (2005). The structure of PhD conclusion chapters.  Journal of English for academic purposes ,  4 (3), 207-224.

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Home » Research Paper Conclusion – Writing Guide and Examples

Research Paper Conclusion – Writing Guide and Examples

Table of Contents

Research Paper Conclusion

Research Paper Conclusion

Definition:

A research paper conclusion is the final section of a research paper that summarizes the key findings, significance, and implications of the research. It is the writer’s opportunity to synthesize the information presented in the paper, draw conclusions, and make recommendations for future research or actions.

The conclusion should provide a clear and concise summary of the research paper, reiterating the research question or problem, the main results, and the significance of the findings. It should also discuss the limitations of the study and suggest areas for further research.

Parts of Research Paper Conclusion

The parts of a research paper conclusion typically include:

Restatement of the Thesis

The conclusion should begin by restating the thesis statement from the introduction in a different way. This helps to remind the reader of the main argument or purpose of the research.

Summary of Key Findings

The conclusion should summarize the main findings of the research, highlighting the most important results and conclusions. This section should be brief and to the point.

Implications and Significance

In this section, the researcher should explain the implications and significance of the research findings. This may include discussing the potential impact on the field or industry, highlighting new insights or knowledge gained, or pointing out areas for future research.

Limitations and Recommendations

It is important to acknowledge any limitations or weaknesses of the research and to make recommendations for how these could be addressed in future studies. This shows that the researcher is aware of the potential limitations of their work and is committed to improving the quality of research in their field.

Concluding Statement

The conclusion should end with a strong concluding statement that leaves a lasting impression on the reader. This could be a call to action, a recommendation for further research, or a final thought on the topic.

How to Write Research Paper Conclusion

Here are some steps you can follow to write an effective research paper conclusion:

  • Restate the research problem or question: Begin by restating the research problem or question that you aimed to answer in your research. This will remind the reader of the purpose of your study.
  • Summarize the main points: Summarize the key findings and results of your research. This can be done by highlighting the most important aspects of your research and the evidence that supports them.
  • Discuss the implications: Discuss the implications of your findings for the research area and any potential applications of your research. You should also mention any limitations of your research that may affect the interpretation of your findings.
  • Provide a conclusion : Provide a concise conclusion that summarizes the main points of your paper and emphasizes the significance of your research. This should be a strong and clear statement that leaves a lasting impression on the reader.
  • Offer suggestions for future research: Lastly, offer suggestions for future research that could build on your findings and contribute to further advancements in the field.

Remember that the conclusion should be brief and to the point, while still effectively summarizing the key findings and implications of your research.

Example of Research Paper Conclusion

Here’s an example of a research paper conclusion:

Conclusion :

In conclusion, our study aimed to investigate the relationship between social media use and mental health among college students. Our findings suggest that there is a significant association between social media use and increased levels of anxiety and depression among college students. This highlights the need for increased awareness and education about the potential negative effects of social media use on mental health, particularly among college students.

Despite the limitations of our study, such as the small sample size and self-reported data, our findings have important implications for future research and practice. Future studies should aim to replicate our findings in larger, more diverse samples, and investigate the potential mechanisms underlying the association between social media use and mental health. In addition, interventions should be developed to promote healthy social media use among college students, such as mindfulness-based approaches and social media detox programs.

Overall, our study contributes to the growing body of research on the impact of social media on mental health, and highlights the importance of addressing this issue in the context of higher education. By raising awareness and promoting healthy social media use among college students, we can help to reduce the negative impact of social media on mental health and improve the well-being of young adults.

Purpose of Research Paper Conclusion

The purpose of a research paper conclusion is to provide a summary and synthesis of the key findings, significance, and implications of the research presented in the paper. The conclusion serves as the final opportunity for the writer to convey their message and leave a lasting impression on the reader.

The conclusion should restate the research problem or question, summarize the main results of the research, and explain their significance. It should also acknowledge the limitations of the study and suggest areas for future research or action.

Overall, the purpose of the conclusion is to provide a sense of closure to the research paper and to emphasize the importance of the research and its potential impact. It should leave the reader with a clear understanding of the main findings and why they matter. The conclusion serves as the writer’s opportunity to showcase their contribution to the field and to inspire further research and action.

When to Write Research Paper Conclusion

The conclusion of a research paper should be written after the body of the paper has been completed. It should not be written until the writer has thoroughly analyzed and interpreted their findings and has written a complete and cohesive discussion of the research.

Before writing the conclusion, the writer should review their research paper and consider the key points that they want to convey to the reader. They should also review the research question, hypotheses, and methodology to ensure that they have addressed all of the necessary components of the research.

Once the writer has a clear understanding of the main findings and their significance, they can begin writing the conclusion. The conclusion should be written in a clear and concise manner, and should reiterate the main points of the research while also providing insights and recommendations for future research or action.

Characteristics of Research Paper Conclusion

The characteristics of a research paper conclusion include:

  • Clear and concise: The conclusion should be written in a clear and concise manner, summarizing the key findings and their significance.
  • Comprehensive: The conclusion should address all of the main points of the research paper, including the research question or problem, the methodology, the main results, and their implications.
  • Future-oriented : The conclusion should provide insights and recommendations for future research or action, based on the findings of the research.
  • Impressive : The conclusion should leave a lasting impression on the reader, emphasizing the importance of the research and its potential impact.
  • Objective : The conclusion should be based on the evidence presented in the research paper, and should avoid personal biases or opinions.
  • Unique : The conclusion should be unique to the research paper and should not simply repeat information from the introduction or body of the paper.

Advantages of Research Paper Conclusion

The advantages of a research paper conclusion include:

  • Summarizing the key findings : The conclusion provides a summary of the main findings of the research, making it easier for the reader to understand the key points of the study.
  • Emphasizing the significance of the research: The conclusion emphasizes the importance of the research and its potential impact, making it more likely that readers will take the research seriously and consider its implications.
  • Providing recommendations for future research or action : The conclusion suggests practical recommendations for future research or action, based on the findings of the study.
  • Providing closure to the research paper : The conclusion provides a sense of closure to the research paper, tying together the different sections of the paper and leaving a lasting impression on the reader.
  • Demonstrating the writer’s contribution to the field : The conclusion provides the writer with an opportunity to showcase their contribution to the field and to inspire further research and action.

Limitations of Research Paper Conclusion

While the conclusion of a research paper has many advantages, it also has some limitations that should be considered, including:

  • I nability to address all aspects of the research: Due to the limited space available in the conclusion, it may not be possible to address all aspects of the research in detail.
  • Subjectivity : While the conclusion should be objective, it may be influenced by the writer’s personal biases or opinions.
  • Lack of new information: The conclusion should not introduce new information that has not been discussed in the body of the research paper.
  • Lack of generalizability: The conclusions drawn from the research may not be applicable to other contexts or populations, limiting the generalizability of the study.
  • Misinterpretation by the reader: The reader may misinterpret the conclusions drawn from the research, leading to a misunderstanding of the findings.

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How to write an excellent thesis conclusion [with examples]

Tips for writing thesis conclusion

Restate the thesis

Review or reiterate key points of your work, explain why your work is relevant, a take-away for the reader, more resources on writing thesis conclusions, frequently asked questions about writing an excellent thesis conclusion, related articles.

At this point in your writing, you have most likely finished your introduction and the body of your thesis, dissertation, or research paper . While this is a reason to celebrate, you should not underestimate the importance of your conclusion. The conclusion is the last thing that your reader will see, so it should be memorable.

A good conclusion will review the key points of the thesis and explain to the reader why the information is relevant, applicable, or related to the world as a whole. Make sure to dedicate enough of your writing time to the conclusion and do not put it off until the very last minute.

This article provides an effective technique for writing a conclusion adapted from Erika Eby’s The College Student's Guide to Writing a Good Research Paper: 101 Easy Tips & Tricks to Make Your Work Stand Out .

While the thesis introduction starts out with broad statements about the topic, and then narrows it down to the thesis statement , a thesis conclusion does the same in the opposite order.

  • Restate the thesis.
  • Review or reiterate key points of your work.
  • Explain why your work is relevant.
  • Include a core take-away message for the reader.

Tip: Don’t just copy and paste your thesis into your conclusion. Restate it in different words.

The best way to start a conclusion is simply by restating the thesis statement. That does not mean just copying and pasting it from the introduction, but putting it into different words.

You will need to change the structure and wording of it to avoid sounding repetitive. Also, be firm in your conclusion just as you were in the introduction. Try to avoid sounding apologetic by using phrases like "This paper has tried to show..."

The conclusion should address all the same parts as the thesis while making it clear that the reader has reached the end. You are telling the reader that your research is finished and what your findings are.

I have argued throughout this work that the point of critical mass for biopolitical immunity occurred during the Romantic period because of that era's unique combination of post-revolutionary politics and innovations in smallpox prevention. In particular, I demonstrated that the French Revolution and the discovery of vaccination in the 1790s triggered a reconsideration of the relationship between bodies and the state.

Tip: Try to reiterate points from your introduction in your thesis conclusion.

The next step is to review the main points of the thesis as a whole. Look back at the body of of your project and make a note of the key ideas. You can reword these ideas the same way you reworded your thesis statement and then incorporate that into the conclusion.

You can also repeat striking quotations or statistics, but do not use more than two. As the conclusion represents your own closing thoughts on the topic , it should mainly consist of your own words.

In addition, conclusions can contain recommendations to the reader or relevant questions that further the thesis. You should ask yourself:

  • What you would ideally like to see your readers do in reaction to your paper?
  • Do you want them to take a certain action or investigate further?
  • Is there a bigger issue that your paper wants to draw attention to?

Also, try to reference your introduction in your conclusion. You have already taken a first step by restating your thesis. Now, check whether there are other key words, phrases or ideas that are mentioned in your introduction that fit into your conclusion. Connecting the introduction to the conclusion in this way will help readers feel satisfied.

I explored how Mary Wollstonecraft, in both her fiction and political writings, envisions an ideal medico-political state, and how other writers like William Wordsworth and Mary Shelley increasingly imagined the body politic literally, as an incorporated political collective made up of bodies whose immunity to political and medical ills was essential to a healthy state.

Tip: Make sure to explain why your thesis is relevant to your field of research.

Although you can encourage readers to question their opinions and reflect on your topic, do not leave loose ends. You should provide a sense of resolution and make sure your conclusion wraps up your argument. Make sure you explain why your thesis is relevant to your field of research and how your research intervenes within, or substantially revises, existing scholarly debates.

This project challenged conventional ideas about the relationship among Romanticism, medicine, and politics by reading the unfolding of Romantic literature and biopolitical immunity as mutual, co-productive processes. In doing so, this thesis revises the ways in which biopolitics has been theorized by insisting on the inherent connections between Romantic literature and the forms of biopower that characterize early modernity.

Tip: If you began your thesis with an anecdote or historical example, you may want to return to that in your conclusion.

End your conclusion with something memorable, such as:

  • a call to action
  • a recommendation
  • a gesture towards future research
  • a brief explanation of how the problem or idea you covered remains relevant

Ultimately, you want readers to feel more informed, or ready to act, as they read your conclusion.

Yet, the Romantic period is only the beginning of modern thought on immunity and biopolitics. Victorian writers, doctors, and politicians upheld the Romantic idea that a "healthy state" was a literal condition that could be achieved by combining politics and medicine, but augmented that idea through legislation and widespread public health measures. While many nineteenth-century efforts to improve citizens' health were successful, the fight against disease ultimately changed course in the twentieth century as global immunological threats such as SARS occupied public consciousness. Indeed, as subsequent public health events make apparent, biopolitical immunity persists as a viable concept for thinking about the relationship between medicine and politics in modernity.

Need more advice? Read our 5 additional tips on how to write a good thesis conclusion.

The conclusion is the last thing that your reader will see, so it should be memorable. To write a great thesis conclusion you should:

The basic content of a conclusion is to review the main points from the paper. This part represents your own closing thoughts on the topic. It should mainly consist of the outcome of the research in your own words.

The length of the conclusion will depend on the length of the whole thesis. Usually, a conclusion should be around 5-7% of the overall word count.

End your conclusion with something memorable, such as a question, warning, or call to action. Depending on the topic, you can also end with a recommendation.

In Open Access: Theses and Dissertations you can find thousands of completed works. Take a look at any of the theses or dissertations for real-life examples of conclusions that were already approved.

examples of conclusion research paper

How to write a strong conclusion for your research paper

Last updated

17 February 2024

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Short on time? Get an AI generated summary of this article instead

Writing a research paper is a chance to share your knowledge and hypothesis. It's an opportunity to demonstrate your many hours of research and prove your ability to write convincingly.

Ideally, by the end of your research paper, you'll have brought your readers on a journey to reach the conclusions you've pre-determined. However, if you don't stick the landing with a good conclusion, you'll risk losing your reader’s trust.

Writing a strong conclusion for your research paper involves a few important steps, including restating the thesis and summing up everything properly.

Find out what to include and what to avoid, so you can effectively demonstrate your understanding of the topic and prove your expertise.

  • Why is a good conclusion important?

A good conclusion can cement your paper in the reader’s mind. Making a strong impression in your introduction can draw your readers in, but it's the conclusion that will inspire them.

  • What to include in a research paper conclusion

There are a few specifics you should include in your research paper conclusion. Offer your readers some sense of urgency or consequence by pointing out why they should care about the topic you have covered. Discuss any common problems associated with your topic and provide suggestions as to how these problems can be solved or addressed.

The conclusion should include a restatement of your initial thesis. Thesis statements are strengthened after you’ve presented supporting evidence (as you will have done in the paper), so make a point to reintroduce it at the end.

Finally, recap the main points of your research paper, highlighting the key takeaways you want readers to remember. If you've made multiple points throughout the paper, refer to the ones with the strongest supporting evidence.

  • Steps for writing a research paper conclusion

Many writers find the conclusion the most challenging part of any research project . By following these three steps, you'll be prepared to write a conclusion that is effective and concise.

  • Step 1: Restate the problem

Always begin by restating the research problem in the conclusion of a research paper. This serves to remind the reader of your hypothesis and refresh them on the main point of the paper. 

When restating the problem, take care to avoid using exactly the same words you employed earlier in the paper.

  • Step 2: Sum up the paper

After you've restated the problem, sum up the paper by revealing your overall findings. The method for this differs slightly, depending on whether you're crafting an argumentative paper or an empirical paper.

Argumentative paper: Restate your thesis and arguments

Argumentative papers involve introducing a thesis statement early on. In crafting the conclusion for an argumentative paper, always restate the thesis, outlining the way you've developed it throughout the entire paper.

It might be appropriate to mention any counterarguments in the conclusion, so you can demonstrate how your thesis is correct or how the data best supports your main points.

Empirical paper: Summarize research findings

Empirical papers break down a series of research questions. In your conclusion, discuss the findings your research revealed, including any information that surprised you.

Be clear about the conclusions you reached, and explain whether or not you expected to arrive at these particular ones.

  • Step 3: Discuss the implications of your research

Argumentative papers and empirical papers also differ in this part of a research paper conclusion. Here are some tips on crafting conclusions for argumentative and empirical papers.

Argumentative paper: Powerful closing statement

In an argumentative paper, you'll have spent a great deal of time expressing the opinions you formed after doing a significant amount of research. Make a strong closing statement in your argumentative paper's conclusion to share the significance of your work.

You can outline the next steps through a bold call to action, or restate how powerful your ideas turned out to be.

Empirical paper: Directions for future research

Empirical papers are broader in scope. They usually cover a variety of aspects and can include several points of view.

To write a good conclusion for an empirical paper, suggest the type of research that could be done in the future, including methods for further investigation or outlining ways other researchers might proceed.

If you feel your research had any limitations, even if they were outside your control, you could mention these in your conclusion.

After you finish outlining your conclusion, ask someone to read it and offer feedback. In any research project you're especially close to, it can be hard to identify problem areas. Having a close friend or someone whose opinion you value read the research paper and provide honest feedback can be invaluable. Take note of any suggested edits and consider incorporating them into your paper if they make sense.

  • Things to avoid in a research paper conclusion

Keep these aspects to avoid in mind as you're writing your conclusion and refer to them after you've created an outline.

Dry summary

Writing a memorable, succinct conclusion is arguably more important than a strong introduction. Take care to avoid just rephrasing your main points, and don't fall into the trap of repeating dry facts or citations.

You can provide a new perspective for your readers to think about or contextualize your research. Either way, make the conclusion vibrant and interesting, rather than a rote recitation of your research paper’s highlights.

Clichéd or generic phrasing

Your research paper conclusion should feel fresh and inspiring. Avoid generic phrases like "to sum up" or "in conclusion." These phrases tend to be overused, especially in an academic context and might turn your readers off.

The conclusion also isn't the time to introduce colloquial phrases or informal language. Retain a professional, confident tone consistent throughout your paper’s conclusion so it feels exciting and bold.

New data or evidence

While you should present strong data throughout your paper, the conclusion isn't the place to introduce new evidence. This is because readers are engaged in actively learning as they read through the body of your paper.

By the time they reach the conclusion, they will have formed an opinion one way or the other (hopefully in your favor!). Introducing new evidence in the conclusion will only serve to surprise or frustrate your reader.

Ignoring contradictory evidence

If your research reveals contradictory evidence, don't ignore it in the conclusion. This will damage your credibility as an expert and might even serve to highlight the contradictions.

Be as transparent as possible and admit to any shortcomings in your research, but don't dwell on them for too long.

Ambiguous or unclear resolutions

The point of a research paper conclusion is to provide closure and bring all your ideas together. You should wrap up any arguments you introduced in the paper and tie up any loose ends, while demonstrating why your research and data are strong.

Use direct language in your conclusion and avoid ambiguity. Even if some of the data and sources you cite are inconclusive or contradictory, note this in your conclusion to come across as confident and trustworthy.

  • Examples of research paper conclusions

Your research paper should provide a compelling close to the paper as a whole, highlighting your research and hard work. While the conclusion should represent your unique style, these examples offer a starting point:

Ultimately, the data we examined all point to the same conclusion: Encouraging a good work-life balance improves employee productivity and benefits the company overall. The research suggests that when employees feel their personal lives are valued and respected by their employers, they are more likely to be productive when at work. In addition, company turnover tends to be reduced when employees have a balance between their personal and professional lives. While additional research is required to establish ways companies can support employees in creating a stronger work-life balance, it's clear the need is there.

Social media is a primary method of communication among young people. As we've seen in the data presented, most young people in high school use a variety of social media applications at least every hour, including Instagram and Facebook. While social media is an avenue for connection with peers, research increasingly suggests that social media use correlates with body image issues. Young girls with lower self-esteem tend to use social media more often than those who don't log onto social media apps every day. As new applications continue to gain popularity, and as more high school students are given smartphones, more research will be required to measure the effects of prolonged social media use.

What are the different kinds of research paper conclusions?

There are no formal types of research paper conclusions. Ultimately, the conclusion depends on the outline of your paper and the type of research you’re presenting. While some experts note that research papers can end with a new perspective or commentary, most papers should conclude with a combination of both. The most important aspect of a good research paper conclusion is that it accurately represents the body of the paper.

Can I present new arguments in my research paper conclusion?

Research paper conclusions are not the place to introduce new data or arguments. The body of your paper is where you should share research and insights, where the reader is actively absorbing the content. By the time a reader reaches the conclusion of the research paper, they should have formed their opinion. Introducing new arguments in the conclusion can take a reader by surprise, and not in a positive way. It might also serve to frustrate readers.

How long should a research paper conclusion be?

There's no set length for a research paper conclusion. However, it's a good idea not to run on too long, since conclusions are supposed to be succinct. A good rule of thumb is to keep your conclusion around 5 to 10 percent of the paper's total length. If your paper is 10 pages, try to keep your conclusion under one page.

What should I include in a research paper conclusion?

A good research paper conclusion should always include a sense of urgency, so the reader can see how and why the topic should matter to them. You can also note some recommended actions to help fix the problem and some obstacles they might encounter. A conclusion should also remind the reader of the thesis statement, along with the main points you covered in the paper. At the end of the conclusion, add a powerful closing statement that helps cement the paper in the mind of the reader.

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Organizing Your Social Sciences Research Paper

  • 9. The Conclusion
  • Purpose of Guide
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The conclusion is intended to help the reader understand why your research should matter to them after they have finished reading the paper. A conclusion is not merely a summary of the main topics covered or a re-statement of your research problem, but a synthesis of key points derived from the findings of your study and, if applicable based on your analysis, explain new areas for future research. For most college-level research papers, two or three well-developed paragraphs is sufficient for a conclusion, although in some cases, more paragraphs may be required in describing the key findings and highlighting their significance.

Conclusions. The Writing Center. University of North Carolina; Conclusions. The Writing Lab and The OWL. Purdue University.

Importance of a Good Conclusion

A well-written conclusion provides important opportunities to demonstrate to the reader your understanding of the research problem. These include:

  • Presenting the last word on the issues you raised in your paper . Just as the introduction gives a first impression to your reader, the conclusion offers a chance to leave a lasting impression. Do this, for example, by highlighting key findings in your analysis that advance new understanding about the research problem, that are unusual or unexpected, or that have important implications applied to practice.
  • Summarizing your thoughts and conveying the larger significance of your study . The conclusion is an opportunity to succinctly re-emphasize  your answer to the "So What?" question by placing the study within the context of how your research advances past studies about the topic.
  • Identifying how a gap in the literature has been addressed . The conclusion can be where you describe how a previously identified gap in the literature [first identified in your literature review section] has been addressed by your research and why this contribution is significant.
  • Demonstrating the importance of your ideas . Don't be shy. The conclusion offers an opportunity to elaborate on the impact and significance of your findings. This is particularly important if your study approached examining the research problem from an unusual or innovative perspective.
  • Introducing possible new or expanded ways of thinking about the research problem . This does not refer to introducing new information [which should be avoided], but to offer new insight and creative approaches for framing or contextualizing the research problem based on the results of your study.

Bunton, David. “The Structure of PhD Conclusion Chapters.” Journal of English for Academic Purposes 4 (July 2005): 207–224; Conclusions. The Writing Center. University of North Carolina; Kretchmer, Paul. Twelve Steps to Writing an Effective Conclusion. San Francisco Edit, 2003-2008; Conclusions. The Writing Lab and The OWL. Purdue University; Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8.

Structure and Writing Style

I.  General Rules

The general function of your paper's conclusion is to restate the main argument . It reminds the reader of your main argument(s) strengths and reiterates the most important evidence supporting those argument(s). Do this by clearly summarizing the context, background, and the necessity of examining the research problem in relation to an issue, controversy, or a gap found in the literature. However, make sure that your conclusion is not simply a repetitive summary of the findings. This reduces the impact of the argument(s) you have developed in your paper.

When writing the conclusion to your paper, follow these general rules:

  • Present your conclusions in clear, concise language. Re-state the purpose of your study, then describe how your findings differ or support those of other studies and why [i.e., describe what were the unique, new, or crucial contributions your study made to the overall research about your topic].
  • Do not simply reiterate your findings or the discussion of your results. Provide a synthesis of arguments presented in the paper to show how these converge to address the research problem and the overall objectives of your study.
  • Indicate opportunities for future research if you haven't already done so in the discussion section of your paper. Highlighting the need for further research provides the reader with evidence that you have an in-depth awareness of the research problem but that further analysis should take place beyond the scope of your investigation.

Consider the following points to help ensure your conclusion is presented well:

  • If the argument or purpose of your paper is complex, you may need to summarize the argument for your reader.
  • If, prior to your conclusion, you have not yet explained the significance of your findings or if you are proceeding inductively, use the end of your paper to describe your main points and explain their significance.
  • Move from a detailed to a general level of consideration that returns the topic to the context provided by the introduction or within a new context that emerges from the data [this is opposite of the introduction, which begins with general discussion of the context and ends with a detailed description of the research problem]. 

The conclusion also provides a place for you to persuasively and succinctly restate the research problem, given that the reader has now been presented with all the information about the topic . Depending on the discipline you are writing in, the concluding paragraph may contain your reflections on the evidence presented. However, the nature of being introspective about the research you have conducted will depend on the topic and whether your professor wants you to express your observations in this way. If asked to think introspectively about the topic, do not delve into idle speculation. Being introspective means looking within yourself as an author to try and understand an issue more deeply, not to guess at possible outcomes or make up scenarios not supported by the evidence.

II.  Developing a Compelling Conclusion

Although an effective conclusion needs to be clear and succinct, it does not need to be written passively or lack a compelling narrative. Strategies to help you move beyond merely summarizing the key points of your research paper may include any of the following:

  • If your paper addresses a critical, contemporary problem, warn readers of the possible consequences of not attending to the problem proactively based on the evidence presented in your study.
  • Recommend a specific course or courses of action that, if adopted, could address a specific problem in practice or in the development of new knowledge leading to positive change.
  • Cite a relevant quotation or expert opinion already noted in your paper in order to lend authority and support to the conclusion(s) you have reached [a good source would be from a source cited in your literature review].
  • Explain the consequences of your research in a way that elicits action or demonstrates urgency in seeking change.
  • Restate a key statistic, fact, or visual image to emphasize the most important finding of your paper.
  • If your discipline encourages personal reflection, illustrate your concluding point by drawing from your own life experiences.
  • Return to an anecdote, an example, or a quotation that you presented in your introduction, but add further insight derived from the findings of your study; use your interpretation of results from your study to recast it in new or important ways.
  • Provide a "take-home" message in the form of a succinct, declarative statement that you want the reader to remember about your study.

III. Problems to Avoid

Failure to be concise Your conclusion section should be concise and to the point. Conclusions that are too lengthy often have unnecessary information in them. The conclusion is not the place for details about your methodology or results. Although you should give a summary of what was learned from your research, this summary should be relatively brief, since the emphasis in the conclusion is on the implications, evaluations, insights, and other forms of analysis that you make. Strategies for writing concisely can be found here .

Failure to comment on larger, more significant issues In the introduction, your task was to move from the general [topic studied within the field of study] to the specific [the research problem]. However, in the conclusion, your task is to move the discussion from specific [your research problem] back to a general discussion framed around the implications and significance of your findings [i.e., how your research contributes new understanding or fills an important gap in the literature]. In short, the conclusion is where you should place your research within a larger context [visualize the structure of your paper as an hourglass--start with a broad introduction and review of the literature, move to the specific method of analysis and the discussion, conclude with a broad summary of the study's implications and significance].

Failure to reveal problems and negative results Negative aspects of the research process should never be ignored. These are problems, deficiencies, or challenges encountered during your study. They should be summarized as a way of qualifying your overall conclusions. If you encountered negative or unintended results [i.e., findings that are validated outside the research context in which they were generated], you must report them in the results section and discuss their implications in the discussion section of your paper. In the conclusion, use negative or surprising results as an opportunity to explain their possible significance and/or how they may form the basis for future research.

Failure to provide a clear summary of what was learned In order to discuss how your research fits within your field of study [and possibly the world at large], you need to summarize briefly and succinctly how it contributes to new knowledge or a new understanding about the research problem. This element of your conclusion may be only a few sentences long, but it often represents the key takeaway for your reader.

Failure to match the objectives of your research Often research objectives in the social and behavioral sciences change while the research is being carried out due to unforeseen factors or unanticipated variables. This is not a problem unless you forget to go back and refine the original objectives in your introduction. As these changes emerge they must be documented so that they accurately reflect what you were trying to accomplish in your research [not what you thought you might accomplish when you began].

Resist the urge to apologize If you've immersed yourself in studying the research problem, you presumably should know a good deal about it [perhaps even more than your professor!]. Nevertheless, by the time you have finished writing, you may be having some doubts about what you have produced. Repress those doubts! Don't undermine your authority as a researcher by saying something like, "This is just one approach to examining this problem; there may be other, much better approaches that...." The overall tone of your conclusion should convey confidence to the reader concerning the validity and realiability of your research.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8; Concluding Paragraphs. College Writing Center at Meramec. St. Louis Community College; Conclusions. The Writing Center. University of North Carolina; Conclusions. The Writing Lab and The OWL. Purdue University; Freedman, Leora  and Jerry Plotnick. Introductions and Conclusions. The Lab Report. University College Writing Centre. University of Toronto; Leibensperger, Summer. Draft Your Conclusion. Academic Center, the University of Houston-Victoria, 2003; Make Your Last Words Count. The Writer’s Handbook. Writing Center. University of Wisconsin Madison; Miquel, Fuster-Marquez and Carmen Gregori-Signes. “Chapter Six: ‘Last but Not Least:’ Writing the Conclusion of Your Paper.” In Writing an Applied Linguistics Thesis or Dissertation: A Guide to Presenting Empirical Research . John Bitchener, editor. (Basingstoke,UK: Palgrave Macmillan, 2010), pp. 93-105; Tips for Writing a Good Conclusion. Writing@CSU. Colorado State University; Kretchmer, Paul. Twelve Steps to Writing an Effective Conclusion. San Francisco Edit, 2003-2008; Writing Conclusions. Writing Tutorial Services, Center for Innovative Teaching and Learning. Indiana University; Writing: Considering Structure and Organization. Institute for Writing Rhetoric. Dartmouth College.

Writing Tip

Don't Belabor the Obvious!

Avoid phrases like "in conclusion...," "in summary...," or "in closing...." These phrases can be useful, even welcome, in oral presentations. But readers can see by the tell-tale section heading and number of pages remaining that they are reaching the end of your paper. You'll irritate your readers if you belabor the obvious.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8.

Another Writing Tip

New Insight, Not New Information!

Don't surprise the reader with new information in your conclusion that was never referenced anywhere else in the paper. This is why the conclusion rarely has citations to sources that haven't been referenced elsewhere in your paper. If you have new information to present, add it to the discussion or other appropriate section of the paper. Note that, although no new information is introduced, the conclusion, along with the discussion section, is where you offer your most "original" contributions in the paper; the conclusion is where you describe the value of your research, demonstrate that you understand the material that you have presented, and position your findings within the larger context of scholarship on the topic, including describing how your research contributes new insights to that scholarship.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8; Conclusions. The Writing Center. University of North Carolina.

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How to Write a Thesis or Dissertation Conclusion

Published on September 6, 2022 by Tegan George and Shona McCombes. Revised on November 20, 2023.

The conclusion is the very last part of your thesis or dissertation . It should be concise and engaging, leaving your reader with a clear understanding of your main findings, as well as the answer to your research question .

In it, you should:

  • Clearly state the answer to your main research question
  • Summarize and reflect on your research process
  • Make recommendations for future work on your thesis or dissertation topic
  • Show what new knowledge you have contributed to your field
  • Wrap up your thesis or dissertation

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Table of contents

Discussion vs. conclusion, how long should your conclusion be, step 1: answer your research question, step 2: summarize and reflect on your research, step 3: make future recommendations, step 4: emphasize your contributions to your field, step 5: wrap up your thesis or dissertation, full conclusion example, conclusion checklist, other interesting articles, frequently asked questions about conclusion sections.

While your conclusion contains similar elements to your discussion section , they are not the same thing.

Your conclusion should be shorter and more general than your discussion. Instead of repeating literature from your literature review , discussing specific research results , or interpreting your data in detail, concentrate on making broad statements that sum up the most important insights of your research.

As a rule of thumb, your conclusion should not introduce new data, interpretations, or arguments.

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Depending on whether you are writing a thesis or dissertation, your length will vary. Generally, a conclusion should make up around 5–7% of your overall word count.

An empirical scientific study will often have a short conclusion, concisely stating the main findings and recommendations for future research. A humanities dissertation topic or systematic review , on the other hand, might require more space to conclude its analysis, tying all the previous sections together in an overall argument.

Your conclusion should begin with the main question that your thesis or dissertation aimed to address. This is your final chance to show that you’ve done what you set out to do, so make sure to formulate a clear, concise answer.

  • Don’t repeat a list of all the results that you already discussed
  • Do synthesize them into a final takeaway that the reader will remember.

An empirical thesis or dissertation conclusion may begin like this:

A case study –based thesis or dissertation conclusion may begin like this:

In the second example, the research aim is not directly restated, but rather added implicitly to the statement. To avoid repeating yourself, it is helpful to reformulate your aims and questions into an overall statement of what you did and how you did it.

Your conclusion is an opportunity to remind your reader why you took the approach you did, what you expected to find, and how well the results matched your expectations.

To avoid repetition , consider writing more reflectively here, rather than just writing a summary of each preceding section. Consider mentioning the effectiveness of your methodology , or perhaps any new questions or unexpected insights that arose in the process.

You can also mention any limitations of your research, but only if you haven’t already included these in the discussion. Don’t dwell on them at length, though—focus on the positives of your work.

  • While x limits the generalizability of the results, this approach provides new insight into y .
  • This research clearly illustrates x , but it also raises the question of y .

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examples of conclusion research paper

You may already have made a few recommendations for future research in your discussion section, but the conclusion is a good place to elaborate and look ahead, considering the implications of your findings in both theoretical and practical terms.

  • Based on these conclusions, practitioners should consider …
  • To better understand the implications of these results, future studies could address …
  • Further research is needed to determine the causes of/effects of/relationship between …

When making recommendations for further research, be sure not to undermine your own work. Relatedly, while future studies might confirm, build on, or enrich your conclusions, they shouldn’t be required for your argument to feel complete. Your work should stand alone on its own merits.

Just as you should avoid too much self-criticism, you should also avoid exaggerating the applicability of your research. If you’re making recommendations for policy, business, or other practical implementations, it’s generally best to frame them as “shoulds” rather than “musts.” All in all, the purpose of academic research is to inform, explain, and explore—not to demand.

Make sure your reader is left with a strong impression of what your research has contributed to the state of your field.

Some strategies to achieve this include:

  • Returning to your problem statement to explain how your research helps solve the problem
  • Referring back to the literature review and showing how you have addressed a gap in knowledge
  • Discussing how your findings confirm or challenge an existing theory or assumption

Again, avoid simply repeating what you’ve already covered in the discussion in your conclusion. Instead, pick out the most important points and sum them up succinctly, situating your project in a broader context.

The end is near! Once you’ve finished writing your conclusion, it’s time to wrap up your thesis or dissertation with a few final steps:

  • It’s a good idea to write your abstract next, while the research is still fresh in your mind.
  • Next, make sure your reference list is complete and correctly formatted. To speed up the process, you can use our free APA citation generator .
  • Once you’ve added any appendices , you can create a table of contents and title page .
  • Finally, read through the whole document again to make sure your thesis is clearly written and free from language errors. You can proofread it yourself , ask a friend, or consider Scribbr’s proofreading and editing service .

Here is an example of how you can write your conclusion section. Notice how it includes everything mentioned above:

V. Conclusion

The current research aimed to identify acoustic speech characteristics which mark the beginning of an exacerbation in COPD patients.

The central questions for this research were as follows: 1. Which acoustic measures extracted from read speech differ between COPD speakers in stable condition and healthy speakers? 2. In what ways does the speech of COPD patients during an exacerbation differ from speech of COPD patients during stable periods?

All recordings were aligned using a script. Subsequently, they were manually annotated to indicate respiratory actions such as inhaling and exhaling. The recordings of 9 stable COPD patients reading aloud were then compared with the recordings of 5 healthy control subjects reading aloud. The results showed a significant effect of condition on the number of in- and exhalations per syllable, the number of non-linguistic in- and exhalations per syllable, and the ratio of voiced and silence intervals. The number of in- and exhalations per syllable and the number of non-linguistic in- and exhalations per syllable were higher for COPD patients than for healthy controls, which confirmed both hypotheses.

However, the higher ratio of voiced and silence intervals for COPD patients compared to healthy controls was not in line with the hypotheses. This unpredicted result might have been caused by the different reading materials or recording procedures for both groups, or by a difference in reading skills. Moreover, there was a trend regarding the effect of condition on the number of syllables per breath group. The number of syllables per breath group was higher for healthy controls than for COPD patients, which was in line with the hypothesis. There was no effect of condition on pitch, intensity, center of gravity, pitch variability, speaking rate, or articulation rate.

This research has shown that the speech of COPD patients in exacerbation differs from the speech of COPD patients in stable condition. This might have potential for the detection of exacerbations. However, sustained vowels rarely occur in spontaneous speech. Therefore, the last two outcome measures might have greater potential for the detection of beginning exacerbations, but further research on the different outcome measures and their potential for the detection of exacerbations is needed due to the limitations of the current study.

Checklist: Conclusion

I have clearly and concisely answered the main research question .

I have summarized my overall argument or key takeaways.

I have mentioned any important limitations of the research.

I have given relevant recommendations .

I have clearly explained what my research has contributed to my field.

I have  not introduced any new data or arguments.

You've written a great conclusion! Use the other checklists to further improve your dissertation.

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In a thesis or dissertation, the discussion is an in-depth exploration of the results, going into detail about the meaning of your findings and citing relevant sources to put them in context.

The conclusion is more shorter and more general: it concisely answers your main research question and makes recommendations based on your overall findings.

While it may be tempting to present new arguments or evidence in your thesis or disseration conclusion , especially if you have a particularly striking argument you’d like to finish your analysis with, you shouldn’t. Theses and dissertations follow a more formal structure than this.

All your findings and arguments should be presented in the body of the text (more specifically in the discussion section and results section .) The conclusion is meant to summarize and reflect on the evidence and arguments you have already presented, not introduce new ones.

For a stronger dissertation conclusion , avoid including:

  • Important evidence or analysis that wasn’t mentioned in the discussion section and results section
  • Generic concluding phrases (e.g. “In conclusion …”)
  • Weak statements that undermine your argument (e.g., “There are good points on both sides of this issue.”)

Your conclusion should leave the reader with a strong, decisive impression of your work.

The conclusion of your thesis or dissertation shouldn’t take up more than 5–7% of your overall word count.

The conclusion of your thesis or dissertation should include the following:

  • A restatement of your research question
  • A summary of your key arguments and/or results
  • A short discussion of the implications of your research

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George, T. & McCombes, S. (2023, November 20). How to Write a Thesis or Dissertation Conclusion. Scribbr. Retrieved June 24, 2024, from https://www.scribbr.com/dissertation/write-conclusion/

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How to Write a Conclusion for a Research Paper

How to Write a Conclusion for a Research Paper

3-minute read

  • 29th August 2023

If you’re writing a research paper, the conclusion is your opportunity to summarize your findings and leave a lasting impression on your readers. In this post, we’ll take you through how to write an effective conclusion for a research paper and how you can:

·   Reword your thesis statement

·   Highlight the significance of your research

·   Discuss limitations

·   Connect to the introduction

·   End with a thought-provoking statement

Rewording Your Thesis Statement

Begin your conclusion by restating your thesis statement in a way that is slightly different from the wording used in the introduction. Avoid presenting new information or evidence in your conclusion. Just summarize the main points and arguments of your essay and keep this part as concise as possible. Remember that you’ve already covered the in-depth analyses and investigations in the main body paragraphs of your essay, so it’s not necessary to restate these details in the conclusion.

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Highlighting the Significance of Your Research

The conclusion is a good place to emphasize the implications of your research . Avoid ambiguous or vague language such as “I think” or “maybe,” which could weaken your position. Clearly explain why your research is significant and how it contributes to the broader field of study.

Here’s an example from a (fictional) study on the impact of social media on mental health:

Discussing Limitations

Although it’s important to emphasize the significance of your study, you can also use the conclusion to briefly address any limitations you discovered while conducting your research, such as time constraints or a shortage of resources. Doing this demonstrates a balanced and honest approach to your research.

Connecting to the Introduction

In your conclusion, you can circle back to your introduction , perhaps by referring to a quote or anecdote you discussed earlier. If you end your paper on a similar note to how you began it, you will create a sense of cohesion for the reader and remind them of the meaning and significance of your research.

Ending With a Thought-Provoking Statement

Consider ending your paper with a thought-provoking and memorable statement that relates to the impact of your research questions or hypothesis. This statement can be a call to action, a philosophical question, or a prediction for the future (positive or negative). Here’s an example that uses the same topic as above (social media and mental health):

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How to Write a Conclusion for a Research Paper

Sumalatha G

Table of Contents

Writing a conclusion for a research paper is a critical step that often determines the overall impact and impression the paper leaves on the reader. While some may view the conclusion as a mere formality, it is actually an opportunity to wrap up the main points, provide closure, and leave a lasting impression. In this article, we will explore the importance of a well-crafted conclusion and discuss various tips and strategies to help you write an engaging and impactful conclusion for your research paper.

Introduction

Before delving into the specifics of writing a conclusion, it is important to understand why it is such a crucial component of a research paper. The conclusion serves to summarize the main points of the paper and reemphasize their significance. A well-written conclusion can leave the reader satisfied and inspired, while a poorly executed one may undermine the credibility of the entire paper. Therefore, it is essential to give careful thought and attention to crafting an effective conclusion.

When writing a research paper, the conclusion acts as the final destination for the reader. It is the point where all the information, arguments, and evidence presented throughout the paper converge. Just as a traveler reaches the end of a journey, the reader reaches the conclusion to find closure and a sense of fulfillment. This is why the conclusion should not be taken lightly; it is a critical opportunity to leave a lasting impact on the reader.

Moreover, the conclusion is not merely a repetition of the introduction or a summary of the main points. It goes beyond that by providing a deeper understanding of the research findings and their implications. It allows the writer to reflect on the significance of their work and its potential contributions to the field. By doing so, the conclusion elevates the research paper from a mere collection of facts to a thought-provoking piece of scholarship.

In the following sections, we will explore various strategies and techniques for crafting a compelling conclusion. By understanding the importance of the conclusion and learning how to write one effectively, you will be equipped to create impactful research papers.

Structuring the Conclusion

In order to create an effective conclusion, it is important to consider its structure. A well-structured conclusion should begin by restating the thesis statement and summarizing the main points of the paper. It should then move on to provide a concise synthesis of the key findings and arguments, highlighting their implications and relevance. Finally, the conclusion should end with a thought-provoking statement that leaves the reader with a lasting impression.

Additionally, using phrases like "this research demonstrates," "the findings show," or "it is clear that" can help to highlight the significance of your research and emphasize your main conclusions.

Tips for Writing an Engaging Conclusion

Writing an engaging conclusion requires careful consideration and attention to detail. Here are some tips to help you create an impactful conclusion for your research paper:

  • Revisit the Introduction: Start your conclusion by referencing your introduction. Remind the reader of the research question or problem you initially posed and show how your research has addressed it.
  • Summarize Your Main Points: Provide a concise summary of the main points and arguments presented in your paper. Be sure to restate your thesis statement and highlight the key findings.
  • Offer a Fresh Perspective: Use the conclusion as an opportunity to provide a fresh perspective or offer insights that go beyond the main body of the paper. This will leave the reader with something new to consider.
  • Leave a Lasting Impression: End your conclusion with a thought-provoking statement or a call to action. This will leave a lasting impression on the reader and encourage further exploration of the research topic.

Addressing Counter Arguments In Conclusion

While crafting your conclusion, you can address any potential counterarguments or limitations of your research. This will demonstrate that you have considered alternative perspectives and have taken them into account in your conclusions. By acknowledging potential counterarguments, you can strengthen the credibility and validity of your research. And by openly discussing limitations, you demonstrate transparency and honesty in your research process.

Language and Tone To Be Used In Conclusion

The language and tone of your conclusion play a crucial role in shaping the overall impression of your research paper. It is important to use clear and concise language that is appropriate for the academic context. Avoid using overly informal or colloquial language that may undermine the credibility of your research. Additionally, consider the tone of your conclusion – it should be professional, confident, and persuasive, while still maintaining a respectful and objective tone.

When it comes to the language used in your conclusion, precision is key. You want to ensure that your ideas are communicated effectively and that there is no room for misinterpretation. Using clear and concise language will not only make your conclusion easier to understand but will also demonstrate your command of the subject matter.

Furthermore, it is important to strike the right balance between formality and accessibility. While academic writing typically requires a more formal tone, you should still aim to make your conclusion accessible to a wider audience. This means avoiding jargon or technical terms that may confuse readers who are not familiar with the subject matter. Instead, opt for language that is clear and straightforward, allowing anyone to grasp the main points of your research.

Another aspect to consider is the tone of your conclusion. The tone should reflect the confidence you have in your research findings and the strength of your argument. By adopting a professional and confident tone, you are more likely to convince your readers of the validity and importance of your research. However, it is crucial to strike a balance and avoid sounding arrogant or dismissive of opposing viewpoints. Maintaining a respectful and objective tone will help you engage with your audience in a more persuasive manner.

Moreover, the tone of your conclusion should align with the overall tone of your research paper. Consistency in tone throughout your paper will create a cohesive and unified piece of writing.

Common Mistakes to Avoid While Writing a Conclusion

When writing a conclusion, there are several common mistakes that researchers often make. By being aware of these pitfalls, you can avoid them and create a more effective conclusion for your research paper. Some common mistakes include:

  • Repeating the Introduction: A conclusion should not simply be a reworded version of the introduction. While it is important to revisit the main points, try to present them in a fresh and broader perspective, by foregrounding the implications/impacts of your research.
  • Introducing New Information: The conclusion should not introduce any new information or arguments. Instead, it should focus on summarizing and synthesizing the main points presented in the paper.
  • Being Vague or General: Avoid using vague or general statements in your conclusion. Instead, be specific and provide concrete examples or evidence to support your main points.
  • Ending Abruptly: A conclusion should provide a sense of closure and completeness. Avoid ending your conclusion abruptly or leaving the reader with unanswered questions.

Editing and Revising the Conclusion

Just like the rest of your research paper, the conclusion should go through a thorough editing and revising process. This will help to ensure clarity, coherence, and impact in the conclusion. As you revise your conclusion, consider the following:

  • Check for Consistency: Ensure that your conclusion aligns with the main body of the paper and does not introduce any new or contradictory information.
  • Eliminate Redundancy: Remove any repetitive or redundant information in your conclusion. Instead, focus on presenting the key points in a concise and engaging manner.
  • Proofread for Clarity: Read your conclusion aloud or ask someone else to read it to ensure that it is clear and understandable. Check for any grammatical or spelling errors that may distract the reader.
  • Seek Feedback: Consider sharing your conclusion with peers or mentors to get their feedback and insights. This can help you strengthen your conclusion and make it more impactful.

How to Write Conclusion as a Call to Action

Finally, consider using your conclusion as a call to action. Encourage the reader to take further action, such as conducting additional research or considering the implications of your findings. By providing a clear call to action, you can inspire the reader to actively engage with your research and continue the conversation on the topic.

Adapting to Different Research Paper Types

It is important to adapt your conclusion approach based on the type of research paper you are writing. Different research paper types may require different strategies and approaches to writing the conclusion. For example, a scientific research paper may focus more on summarizing the key findings and implications, while a persuasive research paper may emphasize the call to action and the potential impact of the research. Tailor your conclusion to suit the specific goals and requirements of your research paper.

Final Thoughts

A well-crafted conclusion can leave a lasting impression on the reader and enhance the impact of your research. By following the tips and strategies outlined in this article, you can create an engaging and impactful conclusion that effectively summarizes your main points, addresses potential counterarguments, and leaves the reader with a sense of closure and inspiration. Embrace the importance of the conclusion and view it as an opportunity to showcase the significance and relevance of your research.

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In a short paper—even a research paper—you don’t need to provide an exhaustive summary as part of your conclusion. But you do need to make some kind of transition between your final body paragraph and your concluding paragraph. This may come in the form of a few sentences of summary. Or it may come in the form of a sentence that brings your readers back to your thesis or main idea and reminds your readers where you began and how far you have traveled.

So, for example, in a paper about the relationship between ADHD and rejection sensitivity, Vanessa Roser begins by introducing readers to the fact that researchers have studied the relationship between the two conditions and then provides her explanation of that relationship. Here’s her thesis: “While socialization may indeed be an important factor in RS, I argue that individuals with ADHD may also possess a neurological predisposition to RS that is exacerbated by the differing executive and emotional regulation characteristic of ADHD.”

In her final paragraph, Roser reminds us of where she started by echoing her thesis: “This literature demonstrates that, as with many other conditions, ADHD and RS share a delicately intertwined pattern of neurological similarities that is rooted in the innate biology of an individual’s mind, a connection that cannot be explained in full by the behavioral mediation hypothesis.”  

Highlight the “so what”  

At the beginning of your paper, you explain to your readers what’s at stake—why they should care about the argument you’re making. In your conclusion, you can bring readers back to those stakes by reminding them why your argument is important in the first place. You can also draft a few sentences that put those stakes into a new or broader context.

In the conclusion to her paper about ADHD and RS, Roser echoes the stakes she established in her introduction—that research into connections between ADHD and RS has led to contradictory results, raising questions about the “behavioral mediation hypothesis.”

She writes, “as with many other conditions, ADHD and RS share a delicately intertwined pattern of neurological similarities that is rooted in the innate biology of an individual’s mind, a connection that cannot be explained in full by the behavioral mediation hypothesis.”  

Leave your readers with the “now what”  

After the “what” and the “so what,” you should leave your reader with some final thoughts. If you have written a strong introduction, your readers will know why you have been arguing what you have been arguing—and why they should care. And if you’ve made a good case for your thesis, then your readers should be in a position to see things in a new way, understand new questions, or be ready for something that they weren’t ready for before they read your paper.

In her conclusion, Roser offers two “now what” statements. First, she explains that it is important to recognize that the flawed behavioral mediation hypothesis “seems to place a degree of fault on the individual. It implies that individuals with ADHD must have elicited such frequent or intense rejection by virtue of their inadequate social skills, erasing the possibility that they may simply possess a natural sensitivity to emotion.” She then highlights the broader implications for treatment of people with ADHD, noting that recognizing the actual connection between rejection sensitivity and ADHD “has profound implications for understanding how individuals with ADHD might best be treated in educational settings, by counselors, family, peers, or even society as a whole.”

To find your own “now what” for your essay’s conclusion, try asking yourself these questions:

  • What can my readers now understand, see in a new light, or grapple with that they would not have understood in the same way before reading my paper? Are we a step closer to understanding a larger phenomenon or to understanding why what was at stake is so important?  
  • What questions can I now raise that would not have made sense at the beginning of my paper? Questions for further research? Other ways that this topic could be approached?  
  • Are there other applications for my research? Could my questions be asked about different data in a different context? Could I use my methods to answer a different question?  
  • What action should be taken in light of this argument? What action do I predict will be taken or could lead to a solution?  
  • What larger context might my argument be a part of?  

What to avoid in your conclusion  

  • a complete restatement of all that you have said in your paper.  
  • a substantial counterargument that you do not have space to refute; you should introduce counterarguments before your conclusion.  
  • an apology for what you have not said. If you need to explain the scope of your paper, you should do this sooner—but don’t apologize for what you have not discussed in your paper.  
  • fake transitions like “in conclusion” that are followed by sentences that aren’t actually conclusions. (“In conclusion, I have now demonstrated that my thesis is correct.”)
  • picture_as_pdf Conclusions

examples of conclusion research paper

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Writing a Paper: Conclusions

Writing a conclusion.

A conclusion is an important part of the paper; it provides closure for the reader while reminding the reader of the contents and importance of the paper. It accomplishes this by stepping back from the specifics in order to view the bigger picture of the document. In other words, it is reminding the reader of the main argument. For most course papers, it is usually one paragraph that simply and succinctly restates the main ideas and arguments, pulling everything together to help clarify the thesis of the paper. A conclusion does not introduce new ideas; instead, it should clarify the intent and importance of the paper. It can also suggest possible future research on the topic.

An Easy Checklist for Writing a Conclusion

It is important to remind the reader of the thesis of the paper so he is reminded of the argument and solutions you proposed.
Think of the main points as puzzle pieces, and the conclusion is where they all fit together to create a bigger picture. The reader should walk away with the bigger picture in mind.
Make sure that the paper places its findings in the context of real social change.
Make sure the reader has a distinct sense that the paper has come to an end. It is important to not leave the reader hanging. (You don’t want her to have flip-the-page syndrome, where the reader turns the page, expecting the paper to continue. The paper should naturally come to an end.)
No new ideas should be introduced in the conclusion. It is simply a review of the material that is already present in the paper. The only new idea would be the suggesting of a direction for future research.

Conclusion Example

As addressed in my analysis of recent research, the advantages of a later starting time for high school students significantly outweigh the disadvantages. A later starting time would allow teens more time to sleep--something that is important for their physical and mental health--and ultimately improve their academic performance and behavior. The added transportation costs that result from this change can be absorbed through energy savings. The beneficial effects on the students’ academic performance and behavior validate this decision, but its effect on student motivation is still unknown. I would encourage an in-depth look at the reactions of students to such a change. This sort of study would help determine the actual effects of a later start time on the time management and sleep habits of students.

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How to Write a Conclusion for a Research Paper

Last Updated: May 8, 2024 Approved

This article was co-authored by Christopher Taylor, PhD . Christopher Taylor is an Adjunct Assistant Professor of English at Austin Community College in Texas. He received his PhD in English Literature and Medieval Studies from the University of Texas at Austin in 2014. wikiHow marks an article as reader-approved once it receives enough positive feedback. This article received 43 testimonials and 83% of readers who voted found it helpful, earning it our reader-approved status. This article has been viewed 2,261,291 times.

The conclusion of a research paper needs to summarize the content and purpose of the paper without seeming too wooden or dry. Every basic conclusion must share several key elements, but there are also several tactics you can play around with to craft a more effective conclusion and several you should avoid to prevent yourself from weakening your paper's conclusion. Here are some writing tips to keep in mind when creating a conclusion for your next research paper.

Sample Conclusions

Writing a basic conclusion.

Step 1 Restate the topic.

  • Do not spend a great amount of time or space restating your topic.
  • A good research paper will make the importance of your topic apparent, so you do not need to write an elaborate defense of your topic in the conclusion.
  • Usually a single sentence is all you need to restate your topic.
  • An example would be if you were writing a paper on the epidemiology of infectious disease, you might say something like "Tuberculosis is a widespread infectious disease that affects millions of people worldwide every year."
  • Yet another example from the humanities would be a paper about the Italian Renaissance: "The Italian Renaissance was an explosion of art and ideas centered around artists, writers, and thinkers in Florence."

Step 2 Restate your thesis.

  • A thesis is a narrowed, focused view on the topic at hand.
  • This statement should be rephrased from the thesis you included in your introduction. It should not be identical or too similar to the sentence you originally used.
  • Try re-wording your thesis statement in a way that complements your summary of the topic of your paper in your first sentence of your conclusion.
  • An example of a good thesis statement, going back to the paper on tuberculosis, would be "Tuberculosis is a widespread disease that affects millions of people worldwide every year. Due to the alarming rate of the spread of tuberculosis, particularly in poor countries, medical professionals are implementing new strategies for the diagnosis, treatment, and containment of this disease ."

Step 3 Briefly summarize your main points.

  • A good way to go about this is to re-read the topic sentence of each major paragraph or section in the body of your paper.
  • Find a way to briefly restate each point mentioned in each topic sentence in your conclusion. Do not repeat any of the supporting details used within your body paragraphs.
  • Under most circumstances, you should avoid writing new information in your conclusion. This is especially true if the information is vital to the argument or research presented in your paper.
  • For example, in the TB paper you could summarize the information. "Tuberculosis is a widespread disease that affects millions of people worldwide. Due to the alarming rate of the spread of tuberculosis, particularly in poor countries, medical professionals are implementing new strategies for the diagnosis, treatment, and containment of this disease. In developing countries, such as those in Africa and Southeast Asia, the rate of TB infections is soaring. Crowded conditions, poor sanitation, and lack of access to medical care are all compounding factors in the spread of the disease. Medical experts, such as those from the World Health Organization are now starting campaigns to go into communities in developing countries and provide diagnostic testing and treatments. However, the treatments for TB are very harsh and have many side effects. This leads to patient non-compliance and spread of multi-drug resistant strains of the disease."

Step 4 Add the points up.

  • Note that this is not needed for all research papers.
  • If you already fully explained what the points in your paper mean or why they are significant, you do not need to go into them in much detail in your conclusion. Simply restating your thesis or the significance of your topic should suffice.
  • It is always best practice to address important issues and fully explain your points in the body of your paper. The point of a conclusion to a research paper is to summarize your argument for the reader and, perhaps, to call the reader to action if needed.

Step 5 Make a call to action when appropriate.

  • Note that a call for action is not essential to all conclusions. A research paper on literary criticism, for instance, is less likely to need a call for action than a paper on the effect that television has on toddlers and young children.
  • A paper that is more likely to call readers to action is one that addresses a public or scientific need. Let's go back to our example of tuberculosis. This is a very serious disease that is spreading quickly and with antibiotic-resistant forms.
  • A call to action in this research paper would be a follow-up statement that might be along the lines of "Despite new efforts to diagnose and contain the disease, more research is needed to develop new antibiotics that will treat the most resistant strains of tuberculosis and ease the side effects of current treatments."

Step 6 Answer the “so what” question.

  • For example, if you are writing a history paper, then you might discuss how the historical topic you discussed matters today. If you are writing about a foreign country, then you might use the conclusion to discuss how the information you shared may help readers understand their own country.

Making Your Conclusion as Effective as Possible

Step 1 Stick with a basic synthesis of information.

  • Since this sort of conclusion is so basic, you must aim to synthesize the information rather than merely summarizing it.
  • Instead of merely repeating things you already said, rephrase your thesis and supporting points in a way that ties them all together.
  • By doing so, you make your research paper seem like a "complete thought" rather than a collection of random and vaguely related ideas.

Step 2 Bring things full circle.

  • Ask a question in your introduction. In your conclusion, restate the question and provide a direct answer.
  • Write an anecdote or story in your introduction but do not share the ending. Instead, write the conclusion to the anecdote in the conclusion of your paper.
  • For example, if you wanted to get more creative and put a more humanistic spin on a paper on tuberculosis, you might start your introduction with a story about a person with the disease, and refer to that story in your conclusion. For example, you could say something like this before you re-state your thesis in your conclusion: "Patient X was unable to complete the treatment for tuberculosis due to severe side effects and unfortunately succumbed to the disease."
  • Use the same concepts and images introduced in your introduction in your conclusion. The images may or may not appear at other points throughout the research paper.

Step 3 Close with logic.

  • Include enough information about your topic to back the statement up but do not get too carried away with excess detail.
  • If your research did not provide you with a clear-cut answer to a question posed in your thesis, do not be afraid to indicate as much.
  • Restate your initial hypothesis and indicate whether you still believe it or if the research you performed has begun swaying your opinion.
  • Indicate that an answer may still exist and that further research could shed more light on the topic at hand.

Step 4 Pose a question.

  • This may not be appropriate for all types of research papers. Most research papers, such as one on effective treatment for diseases, will have the information to make the case for a particular argument already in the paper.
  • A good example of a paper that might ask a question of the reader in the ending is one about a social issue, such as poverty or government policy.
  • Ask a question that will directly get at the heart or purpose of the paper. This question is often the same question, or some version of it, that you may have started with when you began your research.
  • Make sure that the question can be answered by the evidence presented in your paper.
  • If desired you can briefly summarize the answer after stating the question. You could also leave the question hanging for the reader to answer, though.

Step 5 Make a suggestion.

  • Even without a call to action, you can still make a recommendation to your reader.
  • For instance, if you are writing about a topic like third-world poverty, you can various ways for the reader to assist in the problem without necessarily calling for more research.
  • Another example would be, in a paper about treatment for drug-resistant tuberculosis, you could suggest donating to the World Health Organization or research foundations that are developing new treatments for the disease.

Avoiding Common Pitfalls

Step 1 Avoid saying

  • These sayings usually sound stiff, unnatural, or trite when used in writing.
  • Moreover, using a phrase like "in conclusion" to begin your conclusion is a little too straightforward and tends to lead to a weak conclusion. A strong conclusion can stand on its own without being labeled as such.

Step 2 Do not wait until the conclusion to state your thesis.

  • Always state the main argument or thesis in the introduction. A research paper is an analytical discussion of an academic topic, not a mystery novel.
  • A good, effective research paper will allow your reader to follow your main argument from start to finish.
  • This is why it is best practice to start your paper with an introduction that states your main argument and to end the paper with a conclusion that re-states your thesis for re-iteration.

Step 3 Leave out new information.

  • All significant information should be introduced in the body of the paper.
  • Supporting evidence expands the topic of your paper by making it appear more detailed. A conclusion should narrow the topic to a more general point.
  • A conclusion should only summarize what you have already stated in the body of your paper.
  • You may suggest further research or a call to action, but you should not bring in any new evidence or facts in the conclusion.

Step 4 Avoid changing the tone of the paper.

  • Most often, a shift in tone occurs when a research paper with an academic tone gives an emotional or sentimental conclusion.
  • Even if the topic of the paper is of personal significance for you, you should not indicate as much in your paper.
  • If you want to give your paper a more humanistic slant, you could start and end your paper with a story or anecdote that would give your topic more personal meaning to the reader.
  • This tone should be consistent throughout the paper, however.

Step 5 Make no apologies.

  • Apologetic statements include phrases like "I may not be an expert" or "This is only my opinion."
  • Statements like this can usually be avoided by refraining from writing in the first-person.
  • Avoid any statements in the first-person. First-person is generally considered to be informal and does not fit with the formal tone of a research paper.

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  • ↑ http://owl.english.purdue.edu/owl/resource/724/04/
  • ↑ http://www.crlsresearchguide.org/18_Writing_Conclusion.asp
  • ↑ http://writing.wisc.edu/Handbook/PlanResearchPaper.html#conclusion
  • ↑ http://writingcenter.unc.edu/handouts/conclusions/
  • ↑ http://writing2.richmond.edu/writing/wweb/conclude.html

About This Article

Christopher Taylor, PhD

To write a conclusion for a research paper, start by restating your thesis statement to remind your readers what your main topic is and bring everything full circle. Then, briefly summarize all of the main points you made throughout your paper, which will help remind your readers of everything they learned. You might also want to include a call to action if you think more research or work needs to be done on your topic by writing something like, "Despite efforts to contain the disease, more research is needed to develop antibiotics." Finally, end your conclusion by explaining the broader context of your topic and why your readers should care about it, which will help them understand why your topic is relevant and important. For tips from our Academic co-author, like how to avoid common pitfalls when writing your conclusion, scroll down! Did this summary help you? Yes No

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How to Write a Research Paper Conclusion Section

examples of conclusion research paper

What is a conclusion in a research paper?

The conclusion in a research paper is the final paragraph or two in a research paper. In scientific papers, the conclusion usually follows the Discussion section , summarizing the importance of the findings and reminding the reader why the work presented in the paper is relevant.

However, it can be a bit confusing to distinguish the conclusion section/paragraph from a summary or a repetition of your findings, your own opinion, or the statement of the implications of your work. In fact, the conclusion should contain a bit of all of these other parts but go beyond it—but not too far beyond! 

The structure and content of the conclusion section can also vary depending on whether you are writing a research manuscript or an essay. This article will explain how to write a good conclusion section, what exactly it should (and should not) contain, how it should be structured, and what you should avoid when writing it.  

Table of Contents:

What does a good conclusion section do, what to include in a research paper conclusion.

  • Conclusion in an Essay
  • Research Paper Conclusion 
  • Conclusion Paragraph Outline and Example
  • What Not to Do When Writing a Conclusion

The conclusion of a research paper has several key objectives. It should:

  • Restate your research problem addressed in the introduction section
  • Summarize your main arguments, important findings, and broader implications
  • Synthesize key takeaways from your study

The specific content in the conclusion depends on whether your paper presents the results of original scientific research or constructs an argument through engagement with previously published sources.

You presented your general field of study to the reader in the introduction section, by moving from general information (the background of your work, often combined with a literature review ) to the rationale of your study and then to the specific problem or topic you addressed, formulated in the form of the statement of the problem in research or the thesis statement in an essay.

In the conclusion section, in contrast, your task is to move from your specific findings or arguments back to a more general depiction of how your research contributes to the readers’ understanding of a certain concept or helps solve a practical problem, or fills an important gap in the literature. The content of your conclusion section depends on the type of research you are doing and what type of paper you are writing. But whatever the outcome of your work is, the conclusion is where you briefly summarize it and place it within a larger context. It could be called the “take-home message” of the entire paper.

What to summarize in the conclusion

Your conclusion section needs to contain a very brief summary of your work , a very brief summary of the main findings of your work, and a mention of anything else that seems relevant when you now look at your work from a bigger perspective, even if it was not initially listed as one of your main research questions. This could be a limitation, for example, a problem with the design of your experiment that either needs to be considered when drawing any conclusions or that led you to ask a different question and therefore draw different conclusions at the end of your study (compared to when you started out).

Once you have reminded the reader of what you did and what you found, you need to go beyond that and also provide either your own opinion on why your work is relevant (and for whom, and how) or theoretical or practical implications of the study , or make a specific call for action if there is one to be made.   

How to Write an Essay Conclusion

Academic essays follow quite different structures than their counterparts in STEM and the natural sciences. Humanities papers often have conclusion sections that are much longer and contain more detail than scientific papers. There are three main types of academic essay conclusions.

Summarizing conclusion

The most typical conclusion at the end of an analytical/explanatory/argumentative essay is a summarizing conclusion . This is, as the name suggests, a clear summary of the main points of your topic and thesis. Since you might have gone through a number of different arguments or subtopics in the main part of your essay, you need to remind the reader again what those were, how they fit into each other, and how they helped you develop or corroborate your hypothesis.

For an essay that analyzes how recruiters can hire the best candidates in the shortest time or on “how starving yourself will increase your lifespan, according to science”, a summary of all the points you discussed might be all you need. Note that you should not exactly repeat what you said earlier, but rather highlight the essential details and present those to your reader in a different way. 

Externalizing conclusion

If you think that just reminding the reader of your main points is not enough, you can opt for an externalizing conclusion instead, that presents new points that were not presented in the paper so far. These new points can be additional facts and information or they can be ideas that are relevant to the topic and have not been mentioned before.

Such a conclusion can stimulate your readers to think about your topic or the implications of your analysis in a whole new way. For example, at the end of a historical analysis of a specific event or development, you could direct your reader’s attention to some current events that were not the topic of your essay but that provide a different context for your findings.

Editorial conclusion

In an editorial conclusion , another common type of conclusion that you will find at the end of papers and essays, you do not add new information but instead present your own experiences or opinions on the topic to round everything up. What makes this type of conclusion interesting is that you can choose to agree or disagree with the information you presented in your paper so far. For example, if you have collected and analyzed information on how a specific diet helps people lose weight, you can nevertheless have your doubts on the sustainability of that diet or its practicability in real life—if such arguments were not included in your original thesis and have therefore not been covered in the main part of your paper, the conclusion section is the place where you can get your opinion across.    

How to Conclude an Empirical Research Paper

An empirical research paper is usually more concise and succinct than an essay, because, if it is written well, it focuses on one specific question, describes the method that was used to answer that one question, describes and explains the results, and guides the reader in a logical way from the introduction to the discussion without going on tangents or digging into not absolutely relevant topics.

Summarize the findings

In a scientific paper, you should include a summary of the findings. Don’t go into great detail here (you will have presented your in-depth  results  and  discussion  already), but do clearly express the answers to the  research questions  you investigated.

Describe your main findings, even if they weren’t necessarily the ones anticipated, and explain the conclusion they led you to. Explain these findings in as few words as possible.

Instead of beginning with “ In conclusion, in this study, we investigated the effect of stress on the brain using fMRI …”, you should try to find a way to incorporate the repetition of the essential (and only the essential) details into the summary of the key points. “ The findings of this fMRI study on the effect of stress on the brain suggest that …” or “ While it has been known for a long time that stress has an effect on the brain, the findings of this fMRI study show that, surprisingly… ” would be better ways to start a conclusion. 

You should also not bring up new ideas or present new facts in the conclusion of a research paper, but stick to the background information you have presented earlier, to the findings you have already discussed, and the limitations and implications you have already described. The one thing you can add here is a practical recommendation that you haven’t clearly stated before—but even that one needs to follow logically from everything you have already discussed in the discussion section.

Discuss the implications

After summing up your key arguments or findings, conclude the paper by stating the broader implications of the research , whether in methods , approach, or findings. Express practical or theoretical takeaways from your paper. This often looks like a “call to action” or a final “sales pitch” that puts an exclamation point on your paper.

If your research topic is more theoretical in nature, your closing statement should express the significance of your argument—for example, in proposing a new understanding of a topic or laying the groundwork for future research.

Future research example

Future research into education standards should focus on establishing a more detailed picture of how novel pedagogical approaches impact young people’s ability to absorb new and difficult concepts. Moreover, observational studies are needed to gain more insight into how specific teaching models affect the retention of relationships and facts—for instance, how inquiry-based learning and its emphasis on lateral thinking can be used as a jumping-off point for more holistic classroom approaches.

Research Conclusion Example and Outline

Let’s revisit the study on the effect of stress on the brain we mentioned before and see what the common structure for a conclusion paragraph looks like, in three steps. Following these simple steps will make it easy for you to wrap everything up in one short paragraph that contains all the essential information: 

One: Short summary of what you did, but integrated into the summary of your findings:

While it has been known for a long time that stress has an effect on the brain, the findings of this fMRI study in 25 university students going through mid-term exams show that, surprisingly, one’s attitude to the experienced stress significantly modulates the brain’s response to it. 

Note that you don’t need to repeat any methodological or technical details here—the reader has been presented with all of these before, they have read your results section and the discussion of your results, and even (hopefully!) a discussion of the limitations and strengths of your paper. The only thing you need to remind them of here is the essential outcome of your work. 

Two: Add implications, and don’t forget to specify who this might be relevant for: 

Students could be considered a specific subsample of the general population, but earlier research shows that the effect that exam stress has on their physical and mental health is comparable to the effects of other types of stress on individuals of other ages and occupations. Further research into practical ways of modulating not only one’s mental stress response but potentially also one’s brain activity (e.g., via neurofeedback training) are warranted.

This is a “research implication”, and it is nicely combined with a mention of a potential limitation of the study (the student sample) that turns out not to be a limitation after all (because earlier research suggests we can generalize to other populations). If there already is a lot of research on neurofeedback for stress control, by the way, then this should have been discussed in your discussion section earlier and you wouldn’t say such studies are “warranted” here but rather specify how your findings could inspire specific future experiments or how they should be implemented in existing applications. 

Three: The most important thing is that your conclusion paragraph accurately reflects the content of your paper. Compare it to your research paper title , your research paper abstract , and to your journal submission cover letter , in case you already have one—if these do not all tell the same story, then you need to go back to your paper, start again from the introduction section, and find out where you lost the logical thread. As always, consistency is key.    

Problems to Avoid When Writing a Conclusion 

  • Do not suddenly introduce new information that has never been mentioned before (unless you are writing an essay and opting for an externalizing conclusion, see above). The conclusion section is not where you want to surprise your readers, but the take-home message of what you have already presented.
  • Do not simply copy your abstract, the conclusion section of your abstract, or the first sentence of your introduction, and put it at the end of the discussion section. Even if these parts of your paper cover the same points, they should not be identical.
  • Do not start the conclusion with “In conclusion”. If it has its own section heading, that is redundant, and if it is the last paragraph of the discussion section, it is inelegant and also not really necessary. The reader expects you to wrap your work up in the last paragraph, so you don’t have to announce that. Just look at the above example to see how to start a conclusion in a natural way.
  • Do not forget what your research objectives were and how you initially formulated the statement of the problem in your introduction section. If your story/approach/conclusions changed because of methodological issues or information you were not aware of when you started, then make sure you go back to the beginning and adapt your entire story (not just the ending). 

Consider Receiving Academic Editing Services

When you have arrived at the conclusion of your paper, you might want to head over to Wordvice AI’s AI Writing Assistant to receive a free grammar check for any academic content. 

After drafting, you can also receive English editing and proofreading services , including paper editing services for your journal manuscript. If you need advice on how to write the other parts of your research paper , or on how to make a research paper outline if you are struggling with putting everything you did together, then head over to the Wordvice academic resources pages , where we have a lot more articles and videos for you.

How to Write a Conclusion for a Research Paper: Examples & Tips

You might be wondering about how to write a conclusion paragraph for a research paper. It may seem like your readers should understand your main arguments by the end, so there is no need for it. However, there are several aspects that prove the importance of a conclusion section in research.

Its first and primary function is, of course, a summary of all the main ideas and evidence in the paper. Sometimes research can be quite lengthy so putting all the thoughts you want to share in one place is very handy. Moreover, the conclusion shows how important your work is and suggests new ways of looking at the problem.

Our guide and research paper conclusion example are here to help you with your assignment!

  • ❗ Importance of a Conclusion
  • 👣 Writing Steps
  • 📑 Conclusion Types & Examples
  • ❌ Common Mistakes

🔗 References

❗ importance of a conclusion in a research paper.

A conclusion intends to remind the readers about the main arguments and findings of the whole paper. However, it also highlights the significance of the work. Both these functions help create a long-lasting, memorable impression from your research paper , so always include this part and try to think of the ways to make it even more effective.

Since having a strong concluding paragraph is so crucial for the overall success, you might want to check out a step-by-step guide on writing it. It can assure you don’t miss any vital moments. Moreover, you need to find out what conclusion type would be the best!

👣 How to Write a Conclusion for a Research Paper Step by Step

Below are only a few simple steps that can allow you to write the most persuasive research paper.


The trick is to make the statement as coherent and short as possible. In only one sentence, there should be information about your topic and its importance.

Do you remember that last sentence of your introduction? Here, you need to paraphrase it so that it is not identical to the original .

It’s time to summarize the main arguments of your research paper. It would be easier if you reread your work and highlight only the most important ideas and evidence. Remember that conclusion is not the place for new information. Therefore, focus only on the points you present in the main body of your paper. The purpose of this brief summary is to remind your readers about the importance of the topic you work on.

Here, you can actually drop a few words about how significant your arguments are. If you explain it elsewhere in your writing, there is no need to go into detail in conclusion. Therefore, a short but meaningful sentence can do its job correctly. You might also try and bring a broader perspective here. For instance, describe how your findings make a difference in a particular field. If there is any confusion, it might help if you try to answer the question: “So what?”

Note that, just like the previous point, this one is optional. If you think it would be appropriate, comment on the need for further research on the topic. Usually, papers addressing scientific and public issues are the most suitable choices for calling readers to action. Moreover, it’s a great place to leave a comment on the unanswered question in the main body if there is any.

📑 Types & Examples of Research Paper Conclusions

You may be assigned to write a persuasive or argumentative paper. Or your professor might ask you to develop an analytical or comparative research paper.

Should you write their conclusions in the same way? The answer is “No”. When you write different types of assignments, you need different conclusions.

Argumentative Research Paper Conclusion

To write an excellent argumentative paper conclusion, you need to highlight the most persuasive and strong arguments you have — no need to add many details. In addition, don’t forget you should include the essential components of the conclusion, such as paraphrasing your thesis statement, which points out your opinion on the chosen topic. If you used a strong thesis statement generator , it won’t be hard to do.

Argumentative Research Paper Conclusion Example

Right now, Earth is facing the issue of the sixth extinction, which causes numerous species to fade every day. There are at least three ideas people might use to prevent their total extinction in the next fifty years. More ways of recycling, new approaches to plastic production, and conservation of species could be life-saving.

Analytical Research Paper Conclusion

First, you should restate your thesis statement and summarize the critical points of your arguments. The main difference between the analytical research paper conclusion and other types is that there should definitely be a highlight of a broader context. It means you can add some meaning to the findings.

Analytical Research Paper Conclusion Example

Elon Musk has made a revolution in the way we pay, drive, and even fly. His ideas come only from the desire to make things easier, but eventually, they change the world. Musk first thought about PayPal when he was a student, and now it’s one of the most popular online payment systems. The same with Tesla cars.

Comparative Research Paper Conclusion

An effective comparative paper conclusion requires some analytical skills. You need to be very careful in looking through facts to clearly formulate your findings. Moreover, the sources need to be trustworthy. And, as usual, you need to add a paraphrased thesis statement and a few words about the importance of your study research.

Comparative Research Paper Conclusion Example

Electric cars are proven to be more efficient and effective than gas cars. Not only do they produce fewer emissions, but the drivers reach their destination point faster. Moreover, gas cars are more expensive to maintain. It all derives from the specifics of the electric cars’ engines, which are much simpler.

❌ Common Mistakes You Should Avoid

Are you ready to pass your writing? Wait! Have you checked it for the most common mistakes? If no, below are several general errors you should avoid.

A weak conclusion leaves your readers unsatisfied and disappointed. When you write uncertain concluding sentences for essays, it also reduces your arguments’ ability to convey its primary thesis and lessens the quality of the paper.
Your essay should make an expression of finished writing, not just a part. So make sure that you expressed your thoughts entirely.
A conclusion should only summarize your research. Include all your findings and arguments in body paragraphs.
A conclusion should be focused and concise. Don’t end an essay with inappropriate or extraneous information.
Some students tend to finish a paper with a single or a few sentences. Moreover, closing sentences for essays merely attempt to revisit the paper’s thesis simplistically or crudely. If you make this mistake, you will give your readers a sense of laziness. Don’t do that! You need a reasonable conclusion, don’t you? Write at least one comprehensive paragraph.
  • The Conclusion – Organizing Academic Research Papers
  • Conclusions – UNC Writing Center
  • Conclusions // Purdue Writing Lab
  • Writing the conclusion – Research & Learning Online
  • Writing a Research Paper – The Writing Center – UW–Madison
  • How to Structure & Organize Your Paper
  • Writing Conclusions: Writing Guides
  • Ending the Essay: Conclusions
  • Tips for Writing Policy Papers – Stanford Law School
  • A Process Approach to Writing Research Papers
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Very, very useful website for students. I appreciate people who kindly share brilliant writing tips with others! Thanks a lot!

Great article! It helped me to complete my research papers conclusion that was a real nightmare for me! Thank you so much for it!

I was on my way to completing my research paper and going to finish the conclusion by copy-pasting the introduction. Thank God, I wanted to read some articles on conclusion writing. After reading your post on this question, I completed my conclusion following your instructions. Believe my research paper is worth an excellent mark!

Custom Writing

In response to a comment from Lynn: Thank you for noting this unfortunate mistake in the text. Obviously, we spend a lot of time compiling, writing, and editing materials, but there’s always room for a simple human error! Thanks again, and have a great day! 🙂

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examples of conclusion research paper

How to Write a Conclusion for an Essay - Tips and Examples

examples of conclusion research paper

The conclusion of your essay is like the grand finale of a fireworks display. It's the last impression you leave on your reader, the moment that ties everything together and leaves them with a lasting impact. 

But for many writers, crafting a conclusion can feel like an afterthought, a hurdle to jump after the excitement of developing the main body of their work. Fear not! This article will equip you with the tools and techniques regarding how to write a conclusion for an essay that effectively summarizes your main points, strengthens your argument, and leaves your reader feeling satisfied and engaged.

What Is a Conclusion

In an essay, the conclusion acts as your final curtain call. It's where you revisit your initial claim (thesis), condense your main supporting arguments, and leave the reader with a lasting takeaway. 

Imagine it as the bridge that connects your ideas to a broader significance. A well-crafted conclusion does more than simply summarize; it elevates your points and offers a sense of closure, ensuring the reader leaves with a clear understanding of your argument's impact. In the next section, you will find conclusion ideas that you could use for your essay.

Please note that our online paper writing service can provide you not only with a stand-alone conclusion but with a fully new composition as well!

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Types of Conclusion

Here's a breakdown of various conclusion types, each serving a distinct purpose:

Technique Description Example
📣 Call to Action Encourage readers to take a specific step. "Let's work together to protect endangered species by supporting conservation efforts."
❓ Provocative Question Spark curiosity with a lingering question. "With artificial intelligence rapidly evolving, will creativity remain a uniquely human trait?"
💡 Universal Insight Connect your argument to a broader truth. "The lessons learned from history remind us that even small acts of courage can inspire change."
🔮 Future Implications Discuss the potential consequences of your topic. "The rise of automation may force us to redefine the concept of work in the coming decades."
🌍 Hypothetical Scenario Use a "what if" scenario to illustrate your point. "Imagine a world where everyone had access to clean water. How would it impact global health?"

How Long Should a Conclusion Be

The ideal length of a conclusion depends on the overall length of your essay, but there are some general guidelines:

  • Shorter Essays (500-750 words): Aim for 3-5 sentences. This ensures you effectively wrap up your points without adding unnecessary content.
  • Medium Essays (750-1200 words): Here, you can expand to 5-8 sentences. This provides more space to elaborate on your concluding thought or call to action.
  • Longer Essays (1200+ words): For these, you can have a conclusion of 8-10 sentences. This allows for a more comprehensive summary or a more nuanced exploration of the future implications or broader significance of your topic.

Here are some additional factors to consider:

  • The complexity of your argument: If your essay explores a multifaceted topic, your conclusion might need to be slightly longer to address all the points adequately.
  • Type of conclusion: A call to action or a hypothetical scenario might require a few extra sentences for elaboration compared to a simple summary.

Remember: The most important aspect is ensuring your conclusion effectively summarizes your main points, leaves a lasting impression, and doesn't feel rushed or tacked on.

Here's a helpful rule of thumb:

  • Keep it proportional: Your conclusion should be roughly 5-10% of your total essay length.

How many sentences should a conclusion be?

Essay Length 📝 Recommended Sentence Range 📏
Shorter Essays (500-750 words) 🎈 3-5 sentences
Medium Essays (750-1200 words) 📚 5-8 sentences
Longer Essays (1200+ words) 🏰 8-10 sentences

Conclusion Transition Words

Transition words for conclusion act like signposts for your reader. They smoothly guide them from the main body of your essay to your closing thoughts, ensuring a clear and logical flow of ideas. Here are some transition words specifically suited for concluding your essay:

Technique 🎯 Examples 📝
Summarizing & Restating 📋
Leaving the Reader with a Lasting Impression 🎨
Looking to the Future 🔮
Leaving the Reader with a Question ❓
Adding Emphasis 💡

Remember, the best transition word will depend on the specific type of conclusion you're aiming for.

How to Write a Conclusion

Every essay or dissertation writer knows that the toughest part of working on a conclusion can be striking the right balance. You want to effectively summarize your main points without redundancy, leaving a lasting impression that feels fresh and impactful, all within a concise and focused section. Here’s a step-by-step guide to help you write a stunning essay conclusion:

How to Write a Conclusion

Restate Your Thesis

Briefly remind your reader of your essay's central claim. This doesn't have to be a word-for-word repetition but a concise restatement that refreshes their memory.

Summarize Key Points

In a few sentences, revisit the main arguments you used to support your thesis. When writing a conclusion, don't get bogged down in details, but offer a high-level overview that reinforces your essay's focus.

Leave a Lasting Impression

This is where your knowledge of how to write a good conclusion can shine! Consider a thought-provoking question, a call to action, or a connection to a broader truth—something that lingers in the reader's mind and resonates beyond the final sentence.

Avoid Introducing New Information

The conclusion paragraph shouldn't introduce entirely new ideas. Stick to wrapping up your existing arguments and leaving a final thought.

Ensure Flow and Readability

Transition smoothly from the main body of your essay to the conclusion. Use transition words like "in conclusion," "finally," or "as a result," and ensure your closing sentences feel natural and well-connected to the rest of your work.

Note that you can simply buy essay at any time and focus on other more important assignments or just enjoy your free time.

Conclusion Paragraph Outline

Here's an outline to help you better understand how to write a conclusion paragraph:

Step 🚶 Description 📝
1. Revisit Your Thesis (1-2 sentences) 🎯
2. Summarize Key Points (1-2 sentences) 🔑
3. Lasting Impression (2-3 sentences) 💡 This is where you leave your reader with a final thought. Choose one or a combination of these options: Urge readers to take a specific action related to your topic. Spark curiosity with a lingering question that encourages further exploration. Connect your arguments to a broader truth or principle. Discuss the potential long-term consequences of your topic. Evoke a strong feeling (sadness, anger, hope) for a lasting impact. Conclude with a relevant quote that reinforces your key points or offers a new perspective.
4. Final Touch (Optional - 1 sentence) 🎀 This is not essential but can be a powerful way to end your essay. Consider a: that summarizes your main point in a memorable way. (simile, metaphor) that leaves a lasting impression. that invites the reader to ponder the topic further.
  • Tailor the length of your conclusion to your essay's overall length (shorter essays: 3-5 sentences, longer essays: 8-10 sentences).
  • Ensure a smooth transition from the main body using transition words.
  • Avoid introducing new information; focus on wrapping up your existing points.
  • Proofread for clarity and ensure your conclusion ties everything together and delivers a final impactful statement.

Read more: Persuasive essay outline . 

Do’s and Don’ts of Essay Conclusion Writing

According to professional term paper writers , a strong conclusion is essential for leaving a lasting impression on your reader. Here's a list of action items you should and shouldn’t do when writing an essay conclusion:

Dos ✅ Don'ts ❌
Restate your thesis in a new way. 🔄 Remind the reader of your central claim, but rephrase it to avoid redundancy. Simply repeat your thesis word-for-word. This lacks originality and doesn't offer a fresh perspective.
Summarize your key points concisely. 📝 Briefly revisit the main arguments used to support your thesis. Rehash every detail from your essay. 🔍 Focus on a high-level overview to reinforce your essay's main points.
Leave a lasting impression. 💡 Spark curiosity with a question, propose a call to action, or connect your arguments to a broader truth. End with a bland statement. 😐 Avoid generic closings like "In conclusion..." or "This is important because...".
Ensure a smooth transition. 🌉 Use transition words like "finally," "as a result," or "in essence" to connect your conclusion to the main body. Introduce entirely new information. ⚠️ The conclusion should wrap up existing arguments, not introduce new ideas.
Proofread for clarity and flow. 🔍 Ensure your conclusion feels natural and well-connected to the rest of your work. Leave grammatical errors or awkward phrasing. 🚫 Edit and revise for a polished final sentence.

Conclusion Examples

A strong conclusion isn't just an afterthought – it's the capstone of your essay. Here are five examples of conclusion paragraphs for essays showcasing different techniques to craft a powerful closing to make your essay stand out.

1. Call to Action: (Essay About the Importance of Recycling)

In conclusion, the environmental impact of our waste is undeniable. We all have a responsibility to adopt sustainable practices. We can collectively make a significant difference by incorporating simple changes like recycling into our daily routines. Join the movement – choose to reuse, reduce, and recycle.

2. Provocative Question: (Essay Exploring the Potential Consequences of Artificial Intelligence)

As artificial intelligence rapidly evolves, it's crucial to consider its impact on humanity. While AI holds immense potential for progress, will it remain a tool for good, or will it eventually surpass human control? This question demands our collective attention, as the decisions we make today will shape the future of AI and its impact on our world.

3. Universal Insight: (Essay Analyzing a Historical Event)

The study of history offers valuable lessons that transcend time. The events of the [insert historical event] remind us that even small acts of defiance can have a ripple effect, inspiring change and ultimately leading to a brighter future. Every voice has the power to make a difference, and courage can be contagious.

4. Future Implications: (Essay Discussing the Rise of Social Media)

Social media's explosive growth has transformed how we connect and consume information. While these platforms offer undeniable benefits, their long-term effects on social interaction, mental health, and political discourse require careful consideration. As social media continues to evolve, we must remain vigilant and ensure it remains a tool for positive connection and not a source of division.

5. Hypothetical Scenario: (Essay Arguing for the Importance of Space Exploration)

Imagine a world where our understanding of the universe is limited to Earth. We miss out on the potential for groundbreaking discoveries in physics, medicine, and our place in the cosmos. By continuing to venture beyond our planet, we push the boundaries of human knowledge and inspire future generations to reach for the stars.

Recommended for reading: Nursing essay examples .

Difference Between Good and Weak Conclusions

Not all conclusions are created equal. A weak ending can leave your reader feeling stranded, unsure of where your essay has taken them. Conversely, writing a conclusion that is strong acts as a landing pad, summarizing your key points and leaving a lasting impression.

⚠️ Weak Conclusion ❓ What's Wrong with It? ✅ Good Conclusion
In conclusion, exercise is good for you. It helps you stay healthy and fit. By incorporating regular exercise into our routines, we boost our physical health and energy levels and enhance our mental well-being and resilience. (Rephrased thesis & highlights benefits.)
This event was very significant and had a big impact on history. The [name of historical event] marked a turning point in [explain the historical period]. Its impact resonates today, influencing [mention specific consequences or ongoing effects]. (Connects to specifics & broader significance.)
Throughout this essay, we've discussed the good and bad sides of social media. While social media offers undeniable benefits like connection and information sharing, its impact on mental health, privacy, and political discourse necessitates responsible use and ongoing discussions about its role in society. (Connects arguments to broader issues & future implications.)

Nailed that essay? Don't blow it with a lame ending! A good conclusion is like the mic drop at the end of a rap song. It reminds the reader of your main points but in a cool new way. Throw in a thought-provoking question, a call to action, or a connection to something bigger, and you'll leave them thinking long after they turn the page.

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How To Write A Conclusion For An Essay?

How to write a good conclusion, how to write a conclusion for a college essay.

Daniel Parker

Daniel Parker

is a seasoned educational writer focusing on scholarship guidance, research papers, and various forms of academic essays including reflective and narrative essays. His expertise also extends to detailed case studies. A scholar with a background in English Literature and Education, Daniel’s work on EssayPro blog aims to support students in achieving academic excellence and securing scholarships. His hobbies include reading classic literature and participating in academic forums.

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is an expert in nursing and healthcare, with a strong background in history, law, and literature. Holding advanced degrees in nursing and public health, his analytical approach and comprehensive knowledge help students navigate complex topics. On EssayPro blog, Adam provides insightful articles on everything from historical analysis to the intricacies of healthcare policies. In his downtime, he enjoys historical documentaries and volunteering at local clinics.

  • Updated writing tips.
  • Added informative tables.
  • Added conclusion example.
  • Added an article conclusion.
  • Essay Conclusions | UMGC. (n.d.). University of Maryland Global Campus. https://www.umgc.edu/current-students/learning-resources/writing-center/writing-resources/writing/essay-conclusions
  • How to Write a Conclusion for an Essay | BestColleges. (n.d.). BestColleges.com. https://www.bestcolleges.com/blog/how-to-write-a-conclusion/
  • Ending the Essay: Conclusions | Harvard College Writing Center. (n.d.). https://writingcenter.fas.harvard.edu/pages/ending-essay-conclusions

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What is an Appendix in a Research Paper? | Aithor

What is an Appendix in a Research Paper? | Aithor

What is an appendix in a research paper?

An appendix is a supplementary section at the end of a research paper after the list of references. It contains more information that helps explain the main ideas in the paper. It's not needed in the main part because it would make the paper too long or go “off-topic." The appendix gives readers more details to help them understand the research better without making the main argument hard to follow.

The length of an appendix can differ depending on what kind of research it is and how much extra information there is. But it shouldn't be more than 10-15% of the total number of pages. An appendix document can have many types of information, like tables, figures, charts, graphs, images, interview notes, survey questions, or anything else that helps support the research findings.

What type of information does a research paper appendix include?

A research paper appendix can include a wide range of supporting materials, such as:

  • Raw data sets or statistical tables that are too extensive for the main text
  • Detailed descriptions of research methodologies, instruments, or protocols
  • Interview transcripts or survey questionnaires
  • Correspondence with research participants or collaborators
  • Visual aids, such as graphs, charts, images, or diagrams
  • Glossaries or abbreviation lists
  • Copies of relevant documents, such as consent forms or legal agreements

The specific content of an appendix depends on the nature of the research and the requirements of the academic discipline or publication venue.

How to structure an appendix

When considering how to write an appendix, follow these guidelines:

  • Place the appendix after the references list, starting on a new page
  • Use a clear and descriptive title, such as "Appendix A: Survey Questionnaire"
  • Organize the content logically and label each item systematically (e.g., Table A1, Figure B2)
  • Refer to each appendix item in the main text using parenthetical citations (e.g., "(see Appendix A)")
  • Use consistent formatting throughout the appendix, following the style guide requirements (e.g., APA, MLA, and Chicago)

Remember, the goal is to make it easy for readers to find and understand the extra information without removing the main points you're making.

General rules for completing appendices

In addition to the basic structure, there are some general rules to follow when making appendices. The exact rules might be a little different depending on the citation style you're using, but here are some common ones:

Appendix APA format

The APA format is the most popular at colleges and universities. When using APA format for your appendices, there are a few specific rules to keep in mind:

  • Use the heading "Appendix" followed by a letter (A, B, C) for each distinct appendix
  • Center the appendix title beneath the heading
  • Arrange appendices in the order they are mentioned in the main text
  • Start each appendix on a new page, regardless of its length
  • Use double spacing and indent the first line of each paragraph
  • Include page numbers and place the appendix after the references list

Appendix MLA format

The rules for MLA format are similar to APA, but the difference is that the MLA appendix should be placed before the list of references. Here are some requirements for MLA format:

  • Place the appendix before the references list
  • Use the heading "Appendix," followed by a letter for each distinct appendix
  • Start each appendix on a new page
  • Use double spacing and a hanging indent for each entry
  • Italicize titles of standalone sources (e.g., books, websites)

Appendix Chicago style

Used when assigned academic papers on History. Here are some requirements for Chicago style:

  • Use the plural heading "Appendices" for multiple appendices
  • Use 12-point Times New Roman font
  • Number pages in the top right corner, starting with "Page 1"
  • Omit the page number on the title page
  • Place appendices before the bibliography

No matter which citation style you use, the most important thing is to be consistent and clear when labeling and referencing your appendices.

How do I refer to an appendix?

To refer to an appendix in the main text, follow these guidelines:

  • Mention each appendix at least once in the text, using a parenthetical citation (e.g., "(see Appendix A)")
  • Capitalize "Appendix" when referring to a specific appendix (e.g., "As shown in Appendix B")
  • Use lowercase when referring to appendices in general (e.g., "The appendices contain additional data")
  • Be consistent in your references throughout the paper

For example:

  • "The survey questionnaire (see Appendix A ) was distributed to 100 participants."
  • " Figure B3 in Appendix B illustrates the relationship between variables X and Y."
  • "More information on the parallel between both eras can be found in Appendix C ."

Remember to consult your chosen style guide for specific formatting requirements and guidelines on how to write an appendix and appendix format.

When you're writing a research paper, an appendix can be a helpful way to provide more information supporting your paper's main ideas. By following some simple rules for organizing and mentioning appendices, you can share extra data and back up your points without making the main part of your paper too long or hard to understand.

Want to make writing your research paper as prestigious as possible? Aithor can help make your life easier! This helpful essay-writing tool uses AI to assist you in creating academic and creative works that meet your specific needs in just a short time.

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This critical role draws out he despite court translators, that facilitates a report in one flow from proceeding, so protects rights non-english speakers.

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The blood microbiome is probably not real.

Gloved hands performing a blood smear.

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Up until recently, if bacteria were detected in your blood you would be in a world of trouble. Blood was long considered to be sterile, meaning free of viable microorganisms like bacteria. When disease-causing bacteria spread to the blood, they can cause a life-threatening septic shock.

But the use of DNA sequencing technology has allowed researchers to more easily detect something that had been reported as early as the late 1960s: bacteria can be found in the blood and not cause disease.

As we begin to map out and understand the complex microbial ecosystem that lives in our gut and elsewhere in the body, we contemplate an important question: is there such a thing as a blood microbiome?

Detecting a fingerprint in the blood

Our large intestine is not sterile; it is teeming with bacteria. But there are parts of the body that were long thought to be devoid of microorganisms. The brain. Bones. A variety of internal fluids, like our synovial fluid and peritoneal fluid. And, importantly, the blood.

Blood is made up of a liquid called plasma filled with red blood cells, whose main function is to carry oxygen to our cells. It also transports white blood cells, important to monitor for and fight off infections, as well as platelets, involved in clotting. 

In the 1960s, a team of Italian researchers published  multiple papers  describing “mycoplasm-like forms”—meaning shapes that look like a particular type of bacteria that often contaminate cells cultured in the lab—in the blood of healthy people. This finding was confirmed in 1977 by a different team, which reported that  four out of the 60 blood samples  they had drawn from healthy volunteers showed bacteria growing in them. These types of tests, however, were rudimentary compared to what we have access to now. In the 2000s, they were mostly supplanted by DNA testing.

While we can sequence the entire DNA of any bacteria found in the blood, the technique most often used is 16S rRNA gene sequencing. I have always admired physicists’ penchant for quirky names: gluons, neutrinos, and charm quarks. Molecular biologists, by comparison, tend to be more sober. Yes, we have genes like  Sonic hedgehog  and proteins called scramblases; usually, though, we have to contend with the dryness of “16S rRNA.” You see, RNA is a molecule with many uses. Messenger RNA (or mRNA) acts as a disposable copy of a gene, a template for the production of a specific protein. Transfer RNA (or tRNA) actually brings the building blocks of a protein to where they are being assembled. And ribosomal RNA (or rRNA) is the main component of the giant protein factories in our cells known as ribosomes. One of its subunits is made up of, among others, a particular string of RNA known as the 16S rRNA.

The cool thing about the gene that codes for this 16S rRNA molecule is that it is very old and it mutates at a slow rate. By reading its precise sequence, scientists can tell which species it belongs to. Most of the studies of the putative blood microbiome use this technique to tell which species of bacteria are present in the blood being tested. The limitation of this test, however, is that dead bacteria have DNA too. The fact that DNA from the 16S rRNA gene of a precise bacterial species was detected in someone’s blood does not mean these bacteria were alive. For there to be a microbiome in the blood, these microorganisms need to live.

Which brings us to another important point of discussion. In order for scientists to agree that a blood microbiome exists, they first need to decide on the definition of a microbiome, and this is still a point of contention. In 2020, while companies were more than happy to sell hyped-up services testing your gut microbiome and claiming to interpret what it meant for your health, actual experts in the field met to agree on just what the word meant. “We are lacking,” they  wrote , “a clear commonly agreed definition of the term ‘microbiome’.” For example, do viruses qualify? A microbiome implies life but viruses live on the edge, pun intended: they have the genetic blueprint for life yet they cannot reproduce on their own.

These experts proposed that the word “microbiome” should refer to the sum of two things: the microbiota, meaning the living microorganisms themselves, and their theatre of activity. It’s like saying that the Earth is not simply the life forms it houses, but also all of their individual components, and the traces they leave behind, and the environmental conditions in which they thrive or die. The microbiome is made up of bacteria and other microorganisms, yes, but also their proteins, lipids, sugars, and DNA and RNA molecules, as well as the signalling molecules and toxins that get exchanged within their theatre. (This is where viruses were sorted, by the way: not as part of the living microbiota but as belonging to the theatre of activity of the microbiome.)

The microbiome is a community, and this community has a distinct habitat.

So, what does the evidence say? Is our blood truly host to a thriving community of microorganisms or is something else going on?

Transient and sporadic

Initial studies of the alleged blood microbiome were  small . The amounts of bacteria that were being reported based on DNA sequencing were tiny. If this microbiome existed, it seemed sparse, more  “asteroid field in real life”  than “asteroid field in the movies.”

An issue looming over this early research is that of contamination. If bacteria are detected in a blood sample, were they really in the blood… or did they contaminate supplies along the way? When blood is drawn, the skin, which has its own microbiome, is punctured. The area is usually swabbed with alcohol to kill bacteria, and the supplies used should be sterile, but suffice to say that from the blood draw to the DNA extraction to the DNA amplification to the sequencing of this DNA, bacteria can be introduced into the system. In fact, it is such common knowledge that certain bacteria are found inside of the laboratory kits used by scientists that this ecosystem has its own name: the kitome. One way to rule out these contaminants is to simultaneously run negative controls alongside samples every step of the way, to make sure that these negative controls are indeed free of bacteria. But early papers rarely reported when controls were used.

Last year, results from what purports to be the largest study ever into the question of whether the blood microbiome exists were  published in  Nature Microbiology . A total of 9,770 healthy individuals were tested. The conclusion? Yes, some bacteria could be found in their blood, but the evidence contradicted the claim of an ecosystem. In 84% of the samples tested, no bacteria were detected. In most of the other samples, only one species was found. In an ecosystem, you would expect to see species appearing together repeatedly, but this was not the case here. And the species they found most often in their samples were known to contaminate these types of laboratory experiments.

So, what were the few bacteria found in the blood and not recognized as contaminants doing there in the first place if they were not part of a healthy microbiome? The authors lean toward an alternative explanation that had been floated for many years: these bacteria are transient. They end up in the blood from other parts of the body, either because of some minor leak or through their active transportation into the blood by agents such as dendritic cells. Like pedestrians wandering off onto the highway, these bacteria do not normally live in the blood but they can be seen there when we look at the right moment.

Putting the diagnostic cart before the horse

This blood microbiome story could end here and simply be an interesting example of scientific research homing in on a curious finding, testing a hypothesis, and ultimately refuting it (or at the very least providing strong evidence against it). But given the incentives of modern research and the social-media spotlight cast on the academic literature, there are two slightly worrying angles here that merit discussion.

Scientists are more and more incentivized to find practical applications for their research. It’s not enough, for example, to study bacteria that survive at incredibly high temperatures; we must be assured that the  DNA replication enzyme  these bacteria possess will one day be used in laboratories all over the world to conduct research, identify criminals, and test samples for the presence of a pandemic-causing coronavirus.

In researching this topic, I came across many papers claiming the existence of “blood microbiome signatures” for certain diseases that are not known to be infectious. We are thus not talking about infections leaking in the blood and causing sepsis. I saw reports of signatures for  cardiovascular disease ,  liver disease ,  heart attacks , even for  gastrointestinal disease  in dogs .  The idea is that these signatures could soon be turned into (profitable) diagnostic tests. The problem, of course, is that these studies are based on the hypothesis that a blood microbiome is real; that its equilibrium can be affected by disease; and that these changes can be reliably detected and interpreted.

But if the blood microbiome is imaginary, we are just chasing ghosts. This is not unlike the time that scientists were publishing signatures of microRNAs in the blood for every possible cancer. When I looked at the published literature in grad school, I realized that the multiple signatures reported for a single cancer  barely overlapped . They were just chance findings. Compare enough variables in a small sample set and you will find what appears to be an association.

My second concern is that the transitory leakage of bacteria into the blood, as evidenced by the recent  Nature Microbiology  paper, will be used as confirmation of a pseudoscientific entity: leaky gut syndrome. At the end of their paper, the researchers  hypothesize  that these bacteria end up in the blood because the integrity of certain barriers in the body are compromised during disease or during periods of stress. The “net” in our gut gets a bit porous, and some of our colon’s bacteria end up in circulation, though they are not causing disease as far as we can tell. A form of leaky gut is known to exist  in certain intestinal diseases , likely to be a consequence and not a cause. But leaky gut syndrome, favoured by non-evidence-based practitioners, does not appear to be real, yet many websites portray it as the one true cause of all diseases, a real epidemic. Nuanced scientific findings have a history of being stolen, distorted, and toyed with by fake doctors to give credence to their pet theories. Though I have yet to see examples of it, I suspect work done on this hypothesized blood microbiome will similarly get weaponized.

You have been warned.

Take-home message: - Our blood was long considered to be sterile, meaning free of viable microbes, unless a dangerous infection leaked into it, causing sepsis - Studies have provided evidence for the presence of bacteria in the blood of some healthy humans, leading to the hypothesis that, much like in our gut, our blood is host to a microbiome - The largest study ever done on the topic provided strong evidence against this hypothesis. It seems that when non-disease-causing bacteria find themselves in our blood, it is temporary and occasional

@CrackedScience

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Acta Crystallographica Section D
Acta Crystallographica
Section D
STRUCTURAL BIOLOGY

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1. Introduction

2. materials and methods, 4. discussion, 5. conclusion and outlook, supporting information.

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research papers \(\def\hfill{\hskip 5em}\def\hfil{\hskip 3em}\def\eqno#1{\hfil {#1}}\)

STRUCTURAL
BIOLOGY

Open Access

Factors affecting macromolecule orientations in thin films formed in cryo-EM

a National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Post, Bellary Road, Bengaluru 560 065, India * Correspondence e-mail: [email protected]

The formation of a vitrified thin film embedded with randomly oriented macromolecules is an essential prerequisite for cryogenic sample electron microscopy. Most commonly, this is achieved using the plunge-freeze method first described nearly 40 years ago. Although this is a robust method, the behaviour of different macromolecules shows great variation upon freezing and often needs to be optimized to obtain an isotropic, high-resolution reconstruction. For a macromolecule in such a film, the probability of encountering the air–water interface in the time between blotting and freezing and adopting preferred orientations is very high. 3D reconstruction using preferentially oriented particles often leads to anisotropic and uninterpretable maps. Currently, there are no general solutions to this prevalent issue, but several approaches largely focusing on sample preparation with the use of additives and novel grid modifications have been attempted. In this study, the effect of physical and chemical factors on the orientations of macromolecules was investigated through an analysis of selected well studied macromolecules, and important parameters that determine the behaviour of proteins on cryo-EM grids were revealed. These insights highlight the nature of the interactions that cause preferred orientations and can be utilized to systematically address orientation bias for any given macromolecule and to provide a framework to design small-molecule additives to enhance sample stability and behaviour.

Keywords: cryo-EM ; thin films ; preferred macromolecular orientation ; surfactants ; temperature .

EMDB references: CRP pentamer with CTAB, EMD-37864 ; CRP decamer with CTAB, EMD-37865 ; PaaZ with CTAB at 4°C, EMD-37866 ; catalase with SLS, EMD-37952 ; spike with CTAB, EMD-37953 ; catalase at 20°C, EMD-37954 ; catalase at 4°C, EMD-37955 ; catalase with CTAB, EMD-37956 ; β-galactosidase, no tag, EMD-39808 ; β-galactosidase, with tag, EMD-39809

PDB references: CRP pentamer with CTAB, 8wv4 ; CRP decamer with CTAB, 8wv5 ; PaaZ with CTAB at 4°C, 8wv6 ; catalase with SLS, 8wzh ; spike with CTAB, 8wzi ; catalase at 20°C, 8wzj ; catalase at 4°C, 8wzk ; catalase with CTAB, 8wzm


Examples of anisotropic cryo-EM maps resulting from orientation bias. The upper panel shows the reference-free 2D class averages of ( ) SARS-CoV-2 spike protein and ( ) human erythrocyte catalase. For the spike protein, preferred bottom views are observed. In the case of catalase, a preference for the top/bottom view is evident. In the lower panel, 3D maps with anisotropic features are shown for the preferred and perpendicular views as labelled. The symmetries applied during reconstruction were 1 and 2 for the spike protein and catalase, respectively.

To achieve this goal, we tested some commonly used surfactants with different properties on a set of five proteins: C-reactive protein (CRP) pentamers, CRP decamers, catalase, PaaZ and spike. In addition, we explored the effect of the presence of the histidine tag for spike and β -galactosidase and of physical factors such as the temperature during the sample-application step for catalase and PaaZ. We also serendipitously observed an effect of the grid hole dimensions of the holey carbon grid on the orientation distribution of catalase and discuss this briefly. Through this analysis, we identified factors that affect and determine the behaviour of the macromolecule on grids before freezing and studied their effects with a focus on the preferred orientation problem. This account highlights the factors that contribute to orientation bias and provides valuable information that can assist in achieving the optimal freezing conditions for any given macromolecule.

2.1. Source of proteins

Human C-reactive protein (catalogue No. C4063) and human erythrocyte catalase (catalogue No. C3556) were obtained from Sigma–Aldrich. The protein samples were either concentrated using an Amicon 100 kDa concentrator or diluted in respective buffers for grid freezing. All detergent stocks were made in ultrapure water and dilutions were made and used on the day of the experiment.

The SARS-CoV-2 S plasmid was a kind gift from the Krammer laboratory at Icahn School of Medicine, Mount Sinai. The spike gene was amplified from the plasmid and subcloned in the BacMam vector with a C-terminal HRV 3C cleavage tag followed by a seven-histidine and twin Strep tag. Bacmid DNA and virus were prepared as described in the Invitrogen Bac-to-Bac manual. After two generations of amplification in Sf9 cells, the V2 virus was used for transfection of HEK293F cells at a density of 2 million per millilitre. Sodium butyrate (4 m M ) was added to enhance the production of protein 8 h post-infection. The medium supernatant containing the secreted spike protein was harvested on day 3 by centrifuging the cells at 150 g for 10 min. The medium was incubated with pre-equilibrated Ni–NTA (Qiagen) beads at room temperature for 1–2 h (1 ml of beads per 200 ml of medium). The Ni–NTA beads were washed with phosphate-buffered saline (PBS) containing 20 m M imidazole, followed by elution with 280 m M imidazole in PBS. The eluted protein was run on SDS–PAGE to assess its purity, further concentrated and injected onto a 24 ml Superdex 200 (Cytiva) size-exclusion column to exchange the buffer to 50 m M Tris pH 8, 200 m M NaCl, 1 m M DTT. To cleave the tag, the eluted fractions from Ni–NTA chromatography were diluted with 50 m M Tris pH 8, 200 m M NaCl, 1 m M DTT and incubated with HRV 3C protease overnight at 4°C, followed by reverse IMAC to obtain the spike protein without tag in the flowthrough. The flowthrough was concentrated using an Amicon 100 kDa concentrator, flash-frozen using liquid nitrogen and stored at −80°C until further use.

2.2. Grid preparation

6.3 µl of the protein was thawed on ice and 0.7 µl of 10× additive (surfactant) stock was added to obtain a final concentration of 1×. This sample was incubated on ice for 2–5 min and then centrifuged at 21 000 g for 20 min. Meanwhile, a Vitrobot Mark IV (Thermo Fisher Scientific) chamber was equilibrated at 20°C (unless stated otherwise) and 100% humidity. Quantifoil 1.2/1.3 or Quantifoil 0.6/1 grids were glow-discharged in a reduced-air environment with a PELCO easiGlow chamber using a standard setting of 25 mA current for 1 min. The grid was mounted on the Vitrobot Mark IV and 3 µl of sample was applied to the grid. A blotting time of 3–4 s, a wait time of 10 s and a blot force of 0 were used to obtain a thin film of the specimen. For data sets where grids were prepared at different temperatures, the protein was incubated on a thermal block at the required temperature for 3–7 min before applying it to the grid. The Vitrobot chamber was maintained at the required temperature and 100% humidity. The blot time, blot force and wait time were kept constant.

2.3. Grid screening and data collection


Summary of the parameters for data sets collected under different conditions

Protein Condition Grid type (Quantifoil) Buffer composition Protein concentration (mg ml ) Detector Box size (pixels) Pixel size
CRP pentamer and decamer No additive 1.2/1.3 20 m Tris pH 8, 280 m NaCl, 5 m CaCl , 0.03% NaN 2.1 Falcon 3 256 1.07
CTAB 1.2/1.3 2.6 Falcon 3 256 1.07
SLS 1.2/1.3 2.6 K2 320 1.08
Tween 20 1.2/1.3 3.6 K2 320 1.08
Tween 80 1.2/1.3 6.8 K2 320 1.08
A8-35 1.2/1.3 3.6 Falcon 3 320 1.07
Catalase No additive, 20°C 1.2/1.3 50 m Tris pH 8 0.625 Falcon 3 256 1.07
CTAB 1.2/1.3 3.4 K2 320 1.08
SLS 1.2/1.3 3.4 Falcon 3 256 1.07
Tween 20 1.2/1.3 3.4 K2 320 1.08
Tween 80 1.2/1.3 4.1 Falcon 3 320 1.07
A8-35 1.2/1.3 3.4 Falcon 3 320 1.07
4°C 1.2/1.3 0.625 Falcon 3 256 1.07
37°C 1.2/1.3 0.625 Falcon 3 320 1.38
4°C 0.6/1 0.625 Falcon 3 320 1.07
20°C 0.6/1 0.625 Falcon 3 256 1.07
PaaZ No additive, 4°C 0.6/1 25 m HEPES pH 7.4, 50 m NaCl 0.8 Falcon 3 320 1.07
No additive, 20°C 0.6/1 0.8 Falcon 3 256 1.07
No additive, 37°C 0.6/1 0.8 Falcon 3 1.38
CTAB, 4°C 0.6/1 0.8 Falcon 3 256 1.07
Spike With tag, no additive 0.6/1 50 m Tris pH 8, 200 m NaCl, 1 m DTT 1 Falcon 3 256 1.07
With tag, with CTAB 0.6/1 1.3 Falcon 3 320 1.07
Without tag, no additive 0.6/1 2 Falcon 3 256 1.07
Without tag, with CTAB 0.6/1 2 Falcon 3 256 1.07
β-Galactosidase With tag, no additive 0.6/1 100 m Tris pH 8, 200 m NaCl, 5 m CaCl 2.5 m MgCl , 2 m β-ME 5 Falcon 3 320 1.07
Without tag, no additive 0.6/1 5 Falcon 3 320 1.07

2.4. Data processing and model refinement

3.1. analysis of preferred views of selected macromolecules.


Representative micrographs, with a few selected particles indicated with red circles, and 2D class averages of the test proteins used in this study. ( ) The C-reactive protein (CRP) pentamer adopts a preferred bottom view, which shows the pentameric arrangement of the monomers. ( ) The CRP decamer adopts a preferred side view, which shows the staggered arrangement of two CRP pentamers stacked on top of each other. The same micrograph is used in ( ) and ( ). ( ) Catalase adopts a preferred top view, as seen in the micrograph and 2D class averages. ( ) SARS-CoV-2 spike adopts a preferred bottom view showing the trimeric arrangement. ( ) PaaZ adopts a preferred side view, as seen in the 2D class averages, and the micrograph shows occasional clumping of hexamers on the grids. ( ) β-Galactosidase with an N-terminal polyhistidine tag adopts a preferred side view, as seen in the 2D class averages, and the micrograph shows aggregation on grids. For the above data sets, the catalase and PaaZ grids were prepared at 4°C and all other grids were prepared at 20°C.

3.2. Surfactants affect macromolecule orientation distributions in a charge-dependent manner


Properties of the surfactants used in this study

Additive Charge Ionic or non-ionic CMC Concentration used Aggregation number Molecular weight (Da) Alkyl-chain length Saturation in alkyl chain
CTAB Positive Ionic 1 m (0.04%) 0.054 m (0.002%) 170 364 16 Saturated
SLS Negative Ionic 14.6 m (0.42%) 1.37 m (0.04%) 293 12 Saturated
Tween 20 Neutral Non-ionic 0.06 m (0.007%) 0.04 m (0.005%) 80 1228 12 Saturated
Tween 80 Neutral Non-ionic 0.012 m (0.002%) 0.038 m (0.005%) 58 1310 18 Unsaturated
A8-35 Negative Ionic NA 0.01% NA ∼9000 NA NA


Comparison of parameters for no-additive and surfactant-additive data sets

Protein Condition No. of particles Resolution (Å) (half-map FSC 0.143) Efficiency of Fourier space coverage Sphericity
CRP pentamer No additive 14601 18 0.78 NA
CTAB 36353 3.3 0.80 0.98
SLS 31699 4.2 0.80 0.97
Tween 20 25674 3.3 0.80 0.86
Tween 80 32330 7.5 0.69 NA
A8-35 26737 10 0.78 NA
CRP decamer No additive 9419 20 0.52 NA
CTAB 25992 3.5 0.85 0.98
SLS 59211 3.7 0.79 0.97
Tween 20 51784 4.0 0.78 0.92
Tween 80 36870 4.2 0.79 0.76
A8-35 104369 3.5 0.78 0.98
Catalase No additive 138000 2.7 0.72 0.96
CTAB 153336 2.8 0.76 0.97
SLS 33241 3.7 0.80 0.98
Tween 20 88395 2.9 0.78 0.98
Tween 80 92163 2.9 0.80 0.96
A8-35 122000 3.1 0.77 0.98
PaaZ No additive 51393 4.0 0.76 0.80
CTAB 89454 2.3 0.75 0.98

Orientation-distribution plots from (Scheres, 2012 ) of proteins upon the addition of surfactants with varying properties to the sample buffer before grid preparation. The reference structures of the respective proteins are generated by creating a surface representation in from models from PDB entries , , and . ( ) Changes in the CRP pentamer orientation distribution upon the addition of surfactants. The distributions are distinct from each other, except for Tween 20 and Tween 80, which have similar distributions. ( ) Changes in the CRP decamer orientation distribution upon addition; all surfactants lead to a similar even orientation distribution. ( ) Changes in the catalase orientation distribution upon the addition of surfactants, where the charged surfactants have distinct distributions (CTAB and SLS) and the neutral surfactants (Tween 20 and Tween 80) and A8-35 show similar distributions. ( ) Changes in PaaZ orientation distributions upon the addition of the cationic CTAB. The effects of SLS and Tween 20 on PaaZ were also tested, but visual inspection of the micrographs ( ) showed no improvement and no data were collected; therefore they are not included (marked by asterisks). The effects of Tween 80 and A8-35 on PaaZ were not tested.

3.3. The presence of a solvent-exposed polyhistidine tag affects protein orientations in thin films


The effect of a polyhistidine affinity tag on the SARS-CoV-2 spike protein and β-galactosidase orientation distributions. The different parameters that are used to analyse the quality of the maps are shown next to the orientation plots. indicates the number of particles used for reconstruction, indicates the final resolution of the map, indicates the sphericity and indicates the efficiency of Fourier space coverage. ( ) The locations of the tags on the protein models are indicated by black stars. The models used as references are PDB entries and for the spike protein and β-galactosidase, respectively. ( ) The orientation-distribution plots of the spike protein change upon removal of the affinity tag, but the change is not sufficient to obtain an isotropic map. The addition of the cationic CTAB further alters the orientations of the spike protein without tag and leads to a more isotropic map. β-Galactosidase enzyme (bottom panel) orientations change upon removal of the affinity tag and lead to an isotropic high-resolution map without any additive. The unsharpened final combined maps are shown in grey in ( ).

3.4. The temperature of the incubation chamber during freezing affects protein orientations


The effect of temperature during cryo-EM sample preparation of catalase and PaaZ. Micrographs, maps, orientation-distribution plots and the different parameters that are used to analyse the quality of the maps are shown. indicates the number of particles used for reconstruction, indicates the final resolution of the map, indicates the sphericity and indicates the efficiency of Fourier space coverage. ( ) Catalase orientation distributions change significantly when grids are blotted at different temperatures in the absence of any additive. ( ) PaaZ orientation distributions change slightly when grids are held and blotted at different temperatures in the absence of any additive. In the case of PaaZ, the condition with grids prepared at 4°C with CTAB as an additive is included for comparison as this combination led to a high-resolution isotropic map.

It is evident from these observations that physical factors, such as the grid-preparation temperature, can affect protein behaviour and should be considered as an important screening condition when dealing with orientation bias along with surfactants.

3.5. High-resolution map of E. coli PaaZ in ice


High-resolution cryo-EM map from PaaZ grids prepared at 4°C with CTAB additive. ( ) Comparison of the half-maps and map-versus-model FSCs of the PaaZ data set. ( ) The six coloured individually and in cartoon representation fitted into the cryo-EM map (transparent grey) of PaaZ. ( ) Electrostatic potential surface representation of the domain-swapped PaaZ dimer with waters modelled and shown as cyan spheres. ( ) ResLog plot of PaaZ with the experimental and theoretical numbers of particles required to reach a particular resolution. indicates the number of particles used for reconstruction, is the resolution and indicates the factor, as estimated by post-processing. 3 symmetry was applied for the reconstruction and the ResLog plot indicates the number of particles used, not the number of asymmetric units averaged.

Supplementary Figures and Tables. DOI: https://doi.org/10.1107/S2059798324005229/rr5238sup1.pdf

Acknowledgements

We acknowledge the National Cryo-EM Facility, Bangalore for data collection, which is supported by the Department of Biotechnology (DBT/PR12422/MED/31/287/2014), and the computing facility in the Bangalore Life Science Cluster. We thank Professor Ramaswamy S and all of the laboratory members for critical reading of the manuscript. KRV is part of the EMBO Global Investigator Network. KRV acknowledges the discussion with Drs Pamela Williams and Judith Reeks, Astex, UK on β -galactosidase and the effect of tags. The authors declare no conflicts of interest.

Funding information

KRV acknowledges the support of the Department of Atomic Energy, Government of India under Project Identification No. RTI4006. SY acknowledges the graduate fellowship from TIFR/NCBS.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence , which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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